Mouse anti-Human CD40 Ligand/CD154/TRAP1 Monoclonal Antibody | anti-CD40LG antibody

CD40 Ligand/CD154/TRAP1 (Activation Marker of T-Lymphocytes)

Cat. Num. AAA23948

• Gene Names: CD40LG; IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: CD40 Ligand/CD154/TRAP1, Antibody; CD40 Ligand/CD154/TRAP1 (Activation Marker of T-Lymphocytes); CD154; gp39; hCD40L; HIGM1; IGM; IMD3; T B cell activating molecule; T BAM; T-cell antigen Gp39; TNF-related activation protein;TrAP; Tumor necrosis factor ligand superfamily member 5 (TNFSF5); anti-CD40LG antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: CD40LG/2763
• Specificity: CD40LG expression is mainly confined to the CD4-positive-T-cell subset. Its expression is induced shortly after T-cell activation and represents an early activation marker of T lymphocytes. CD40 is constitutively expressed mainly on B cells, macrophages, and dendritic cells. The CD40-CD40L pathway has been shown to play multiple functional roles in the healthy immune system. It enhances the antigen-specific T-cell response through the activation of dendritic cells and the induction of interleukin-12 production. For example, engagement of CD40 on endothelial cells by activated T cells expressing CD40L leads to upregulation of adhesion molecules such as ICAM-1, VCAM-1, and E-selectin. Activation of APC by CD40-CD40L interaction induces the production of inflammatory cytokines, chemokines, NO, and metalloproteinases. Interaction of CD4-positiveCD40LG-positiveT cells with CD40 on B cells leads to B-cell differentiation, proliferation, immunoglobulin (Ig) isotype switching, and formation of memory B cells.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 261

• Applicable Applications for anti-CD40LG antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin
• Application Notes: ELISA: For coating, order antibody without BSA; FC/FACS: 1-2ug/million cells; IF: 1-2ug/ml; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: Jurkat cells. Human tonsil, spleen or thymus tissue (IHC).
• Immunogen: Recombinant fragment of human CD40LG protein (around aa 108-261) (exact sequence is proprietary)
• Cellular Localization: Cell Surface. Secreted.
• Hu-chromosome Location: Xq26.3
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Application Data

(Analysis of Protein Array containing more than 19,000 full-length human proteins using CD40 Mouse Ligand Monoclonal Antibody (CD40LG/2763) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

FCM (Flow Cytometry)

(Flow Cytometric Analysis of Jurkat cells using CD40 Ligand Mouse Monoclonal Antibody (CD40LG/2763) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)

IF (Immunofluorescence)

(Immunofluorescence staining of Jurkat cells using CD40 Ligand Mouse Monoclonal Antibody (CD40LG/2763)followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.)

SDS-PAGE

(SDS-PAGE Analysis Purified CD40 Ligand Mouse Monoclonal Antibody (CD40LG/2763). Confirmation of Integrity and Purity of Antibody.)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Spleen stained with CD40 Ligand Mouse Monoclonal Antibody (CD40LG/2763).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Spleen stained with CD40 Ligand Mouse Monoclonal Antibody (CD40LG/2763).)

References

Howland, K. C., et al. 2000. J. Immunol.164:4465-4470.

NCBI and Uniprot Product Information

• NCBI GI #: 4557433
• NCBI GeneID: 959
• NCBI Accession #: NP_000065.1
• NCBI GenBank Nucleotide #: NP_000065.1
• UniProt Accession #: P29965
• Molecular Weight: 36kDa
• NCBI Official Full Name: CD40 ligand
• NCBI Official Synonym Full Names: CD40 ligand
• NCBI Official Symbol: CD40LG
• NCBI Official Synonym Symbols: IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
• NCBI Protein Information: CD40 ligand
• UniProt Protein Name: CD40 ligand
• UniProt Gene Name: CD40LG
• UniProt Synonym Gene Names: CD40L; TNFSF5; TRAP; CD40-L; TRAP
• UniProt Entry Name: CD40L_HUMAN