FCM/FACS (Flow Cytometry)
(FlowcytometryusingtheAnti-CD40antibody1C10(AAA72542). Paraformaldehydefixedmousesplenocyteswerestainedwithanti-unknownspecificityantibodyorthemouseIgG1versionof1C10(AAA72542-1.1,blueline)atadilutionof1:100for1hatRT.Afterwashing,theboundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000andcellsanalyzedusingaFACSCantoflow-cytometer.)
CD40 recombinant antibody
Anti-CD40 [1C10], Rabbit IgG, kappa
Gene Names
Cd40; IGM; p50; Bp50; GP39; IMD3; TRAP; HIGM1; T-BAM; Tnfrsf5; AI326936
Reactivity
Mouse
Applications
Immunoprecipitation, Immunofluorescence, ELISA, Flow Cytometry, Functional Assay
Purity
Protein A affinity purified
Synonyms
CD40, Recombinant Antibody; Anti-CD40 [1C10], Rabbit IgG, kappa; CD40; Anti-CD40 [1C10]; TNFRSF5; B-cell surface antigen CD40; Bp50; CD40L receptor; CDW40; MGC9013; T-cell differentiation antigen; Tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5; CD40 recombinant antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG kappa
Clone Number
1C10
Specificity
This antibody binds mouse CD40, which is a member of the tumor necrosis factor receptor superfamily. CD40 is an integral membrane protein found on the surface of B lymphocytes.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for CD40 recombinant antibody
IP (Immunoprecipitation), IF (Immunofluorescence), ELISA, FCM/FACS (Flow Cytometry), Antagonistic Antibody
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format for improved compatibility with existing reagents assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
FCM/FACS (Flow Cytometry)
(FlowcytometryusingtheAnti-CD40antibody1C10(AAA72542). Paraformaldehydefixedmousesplenocyteswerestainedwithanti-unknownspecificityantibodyorthemouseIgG1versionof1C10(AAA72542-1.1,blueline)atadilutionof1:100for1hatRT.Afterwashing,theboundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000andcellsanalyzedusingaFACSCantoflow-cytometer.)
FCM/FACS (Flow Cytometry)
(FlowcytometryusingtheAnti-CD40antibody1C10(AAA72542). Paraformaldehydefixedmousesplenocyteswerestainedwithanti-unknownspecificityantibodyorthemouseIgG1versionof1C10(AAA72542-1.1,blueline)atadilutionof1:100for1hatRT.Afterwashing,theboundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000andcellsanalyzedusingaFACSCantoflow-cytometer.)
IF (Immunofluorescence)
(ImmunofluorescencestainingofmousesplenocytescellswithAnti-CD40(AAA72542)1C10 ImmunofluorescenceanalysisofparaformaldehydefixedmousesplenocytescellsimmobilizedonShi-fixpluscover-slipsstainedwiththechimericmouseIgG1versionof1C10(AAA72542-1.1)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembranestaininginsubsetofcells.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72542-1.1,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
IF (Immunofluorescence)
(ImmunofluorescencestainingofmousesplenocytescellswithAnti-CD40(AAA72542)1C10 ImmunofluorescenceanalysisofparaformaldehydefixedmousesplenocytescellsimmobilizedonShi-fixpluscover-slipsstainedwiththechimericmouseIgG1versionof1C10(AAA72542-1.1)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembranestaininginsubsetofcells.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72542-1.1,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
WB (Western Blot)
(Western BlotusingAnti-CD40antibody1C10(AAA72542). Mousespleen(A)(2ug/ml),thymus(B)(2ug/ml),mouselymphnode(C)(1ug/ml)andlung(D)(1ug/ml)tissuelysates(35ugproteininRIPAbuffer)wereresolvedonaSDSPAGEgelandblotswereprobedwiththechimericmouseversionof1C10(AAA72542-1.1)beforedetectionusingananti-mousesecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.)
WB (Western Blot)
(Western BlotusingAnti-CD40antibody1C10(AAA72542). Mousespleen(A)(2ug/ml),thymus(B)(2ug/ml),mouselymphnode(C)(1ug/ml)andlung(D)(1ug/ml)tissuelysates(35ugproteininRIPAbuffer)wereresolvedonaSDSPAGEgelandblotswereprobedwiththechimericmouseversionof1C10(AAA72542-1.1)beforedetectionusingananti-mousesecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.)
Related Product Information for CD40 recombinant antibody
The original antibody was generated by immunizing a rat with 20 ug of soluble extracellular domain of recombinant murine CD40 (sCD40).
References
Heath et al. Monoclonal antibodies to murine CD40 define two distinct functional epitopes. Eur J Immunol. 1994 Aug;24(8):1828-34. N/A7519997
Note on publication:
This paper discusses the generation and functional characterization of two antibodies that recognize two distinct CD40 epitopes.
Note on publication:
This paper discusses the generation and functional characterization of two antibodies that recognize two distinct CD40 epitopes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
28,878 Da
NCBI Official Full Name
Tumor necrosis factor receptor superfamily member 5
NCBI Official Synonym Full Names
CD40 antigen
NCBI Official Symbol
Cd40
NCBI Official Synonym Symbols
IGM; p50; Bp50; GP39; IMD3; TRAP; HIGM1; T-BAM; Tnfrsf5; AI326936
NCBI Protein Information
tumor necrosis factor receptor superfamily member 5; CD40L receptor; B-cell surface antigen CD40; T-cell differentiation antigen; tumor necrosis factor receptor superfamily, member 5
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 5
UniProt Gene Name
Cd40
UniProt Synonym Gene Names
Tnfrsf5
UniProt Entry Name
TNR5_MOUSE
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Product Notes
The CD40 cd40 (Catalog #AAA72542) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD40 [1C10], Rabbit IgG, kappa reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD40 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), ELISA, FCM/FACS (Flow Cytometry), Antagonistic Antibody. Researchers should empirically determine the suitability of the CD40 cd40 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD40, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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