IF (Immunofluorescence)
(Immunofluorescence staining of fixed Daudi cells with anti-CD40 antibody A54 scFv Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of A54 scFv (AAA72042) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72042, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
CD40 recombinant antibody
Anti-CD40 [A54 scFv]
Gene Names
CD40; p50; Bp50; CDW40; TNFRSF5
Reactivity
Human
Applications
ELISA
Purity
Protein A Affinity Purified
Synonyms
CD40, Recombinant Antibody; Anti-CD40 [A54 scFv]; TNFRSF5; A54; Tumor necrosis factor receptor superfamily member 5; B cell surface antigen CD40; B-cell cell surface antigen CD40; B-cell surface antigen CD40; antigen CD40; CD40 antigen; Bp50; Bp-50; CD40L receptor CDw40; CD40 recombinant antibody
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG lambda
Clone Number
A54 scFv
Specificity
A54 binds specifically to the D1 domain of CD40 (and can only bind to the intact i.e. non-truncated version of CD40 and when an AD2 peptide tag has not been added) and can recognise CD40 on a B cell line (BJAB). This scFv binds to CD40 (immobilized by amine coupling) with a KD within the range of 40nM. CD40 is a glycoprotein of the TNFR superfamily and is expressed on all mature B cells, dendritic cells, activated monocytes, some endothelial cells and some epithelium including the thymus. CD40 binds to the membrane protein CD154 of the TNF superfamily and this interaction is required for the seconfary immune responses and germinal centre formation.
Purity/Purification
Protein A Affinity Purified
Form/Format
PBS only with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Sequence Length
277
Applicable Applications for CD40 recombinant antibody
Blocking, ELISA
Immunogen
A54 human anti-CD40 scFv was selected from the n-CoDeR phage display library after 4 rounds of selections using biotinylated CD40-Fcgamma fusion protein as bait and subsequent capture of bound phages on streptavidin-caoted magnetic beads.
Clone Note
A54 is able to block the CD40-CD154 interaction by around 60-80% and can also induce CD40 sinalling in human B cells when at 'unusually high concentrations' (The CD40 receptor - target, tool and technology, Ellmark, 2002). This scFv can also be used for ELISA studies (PMID: 12153507)
Preparation and Storage
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
IF (Immunofluorescence)
(Immunofluorescence staining of fixed Daudi cells with anti-CD40 antibody A54 scFv Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of A54 scFv (AAA72042) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72042, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of fixed Daudi cells with anti-CD40 antibody A54 scFv Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of A54 scFv (AAA72042) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72042, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Related Product Information for CD40 recombinant antibody
This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
Product Categories/Family for CD40 recombinant antibody
References
Ellmark et al. In vitro molecular evolution of antibody genes mimicking receptor revision Mol Immunol. 2002 Oct;39(5-6):349-56. PMID:12220892
Original Published Journal: Mol Immunol. 2002 Oct;39(5-6):349-56.
Original Published Journal: Mol Immunol. 2002 Oct;39(5-6):349-56.
Publication Note: Describes the creation of an interative library based on 4 similar scFv clones (of which one is A54) using the CDR shiffling principle.
Original Published Journal: Mol Immunol. 2002 Oct;39(5-6):349-56.
Original Published Journal: Mol Immunol. 2002 Oct;39(5-6):349-56.
Publication Note: Describes the creation of an interative library based on 4 similar scFv clones (of which one is A54) using the CDR shiffling principle.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 5 isoform 1
NCBI Official Synonym Full Names
CD40 molecule
NCBI Official Symbol
CD40
NCBI Official Synonym Symbols
p50; Bp50; CDW40; TNFRSF5
NCBI Protein Information
tumor necrosis factor receptor superfamily member 5
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 5
UniProt Gene Name
CD40
UniProt Synonym Gene Names
TNFRSF5
UniProt Entry Name
TNR5_HUMAN
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Product Notes
The CD40 cd40 (Catalog #AAA72042) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD40 [A54 scFv] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD40 can be used in a range of immunoassay formats including, but not limited to, Blocking, ELISA. Researchers should empirically determine the suitability of the CD40 cd40 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD40, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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