Application Data
(Figure A. StarBright Yellow 575 conjugated Mouse anti Human CD19 and FITC conjugated Mouse IgG1 isotype control . Figure B. StarBright Yellow 575 conjugated Mouse anti Human CD19 and FITC conjugated Mouse anti Human CD21 . All experiments performed on red cell lysed human blood gated on single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
Mouse CD44 Monoclonal Antibody | anti-CD44 antibody
MOUSE ANTI HUMAN CD44:FITC
Reactivity
Human, Cat, Baboon, African green monkey
Applications
Flow Cytometry, Functional Assay
Purity
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Synonyms
CD44, Antibody; MOUSE ANTI HUMAN CD44:FITC; H-CAM; PGP-1; anti-CD44 antibody
Host
Mouse
Reactivity
Human, Cat, Baboon, African green monkey
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
156-3C11
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Form/Format
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
ALEXA FLUOR 647, FITC, Purified, RPE
Phosphate buffered saline
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
ALEXA FLUOR 647, FITC, Purified, RPE
Phosphate buffered saline
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Concentration
IgG concentration 0.1mg/ml (varies by lot)
Applicable Applications for anti-CD44 antibody
FCM/FACS (Flow Cytometry)
Immunogen
Stimulated human leucocytes.
Preparation and Storage
IgG concentration 0.1mg/ml
Application Data
(Figure A. StarBright Yellow 575 conjugated Mouse anti Human CD19 and FITC conjugated Mouse IgG1 isotype control . Figure B. StarBright Yellow 575 conjugated Mouse anti Human CD19 and FITC conjugated Mouse anti Human CD21 . All experiments performed on red cell lysed human blood gated on single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
Application Data
(Figure A. StarBright Yellow 575 conjugated Mouse anti Human CD19 and FITC conjugated Mouse IgG1 isotype control . Figure B. StarBright Yellow 575 conjugated Mouse anti Human CD19 and FITC conjugated Mouse anti Human CD21 . All experiments performed on red cell lysed human blood gated on single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
Application Data
(Mouse anti Human CD21 antibody, clone Bu33 used to stain formalin fixed, paraffin embedded human spleen sections by immunohistochemistry, visualized by biotinylated anti-Mouse IgG and streptavidin alkaline phosphatase (red). Nuclei were counterstained with hematoxylin (blue).)
Application Data
(Mouse anti Human CD21 antibody, clone Bu33 used to stain formalin fixed, paraffin embedded human spleen sections by immunohistochemistry, visualized by biotinylated anti-Mouse IgG and streptavidin alkaline phosphatase (red). Nuclei were counterstained with hematoxylin (blue).)
Related Product Information for anti-CD44 antibody
Mouse anti Human CD44 antibody, clone 156-3C11 recognizes human Phagocytic glycoprotein 1 also known as CD44, HCAM or CD44s. CD44 is a ~90 kDa single pass type I transmembrane glycoprotein. Various isoforms of CD44 exist due to differential expression of exon products form the membrane proximal region of the extracellular domain. Mouse anti Human CD44 antibody, clone 156-3C11 recognizes the ~90 kDa standard form lacking any of the alternative spliced products, the clone is expected to recognize all isoforms of CD44. CD44 is expressed on leucocytes, erythrocytes, white matter of the brain and some epithelial cells of the breast and small intestine. Antibodies produced by clone 156-3C11 recognise epitope 3, defined as a protease resistant epitope on the CD44 molecule (CD44 and CD45R Cluster report. In Leucocyte Typing V. White cell differentiation antigens. Eds Schlossman, S.F. et al). CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration (Lesley et al. 1990). CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing (Shimizu et al. 1989). CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential (East and Hart 1993).
Product Categories/Family for anti-CD44 antibody
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Product Notes
The CD44 (Catalog #AAA50483) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI HUMAN CD44:FITC reacts with Human, Cat, Baboon, African green monkey and may cross-react with other species as described in the data sheet. AAA Biotech's CD44 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD44 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD44, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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