Mouse CD44v6 Monoclonal Antibody | anti-CD44v6 antibody
MOUSE ANTI HUMAN CD44v6:FITC
Gene Names
CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
Applications
Flow Cytometry, Functional Assay
Synonyms
CD44v6, Antibody; MOUSE ANTI HUMAN CD44v6:FITC; anti-CD44v6 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
VFF-18
Form/Format
FITC
TRIS buffered saline
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
TRIS buffered saline
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Sequence Length
742
Applicable Applications for anti-CD44v6 antibody
FCM/FACS (Flow Cytometry)
Perservative Stabilisers
0.02% Sodium Azide
1% Bovine Serum Albumin
1% Bovine Serum Albumin
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the P3X63Ag8.653 myeloma cell line.
Immunogen
Glutathione S Transferase (GST) fusion protein corresponding to a variable domain v6 of human CD44.
Target Species
Human
Max Ex/Em
Fluorophore: FITC
Excitation Max(nm): 490
Emission Max(nm): 525
Excitation Max(nm): 490
Emission Max(nm): 525
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Related Product Information for anti-CD44v6 antibody
Mouse anti Human CD44v6, clone VFF-18, specifically recognizes a centrally located 14mer epitope encoded by amino acid residues 18-31, within variant exon 6 (v6) of human CD44 (Reinisch et al, 1998). CD44 is a member of a polymorphic family of transmembrane glycoproteins, which exists as a broadly expressed 80-90kDa standard form (CD44s), and also as numerous splice variants, due to extensive alternative splicing and post-translational modifications.
Many functional properties of CD44s have been determined, including cell adhesion, lymphocyte activation, signal transduction, hematopoiesis, recirculation and homing, and CD44v6 has been particularly implicated in leukocyte to enothelial cell attachment and rolling, as well as homing to sites of inflammation and peripheral organs.
As well as its role as a receptor for hyaluronic acid (HA), CD44s also interacts with other ligands including matrix metalloproteinases(MMPs), fibronectin and collagens, and has been linked with cancer of the breast, colon, stomach and squamous cell carcinomas (HNSCC) of the head and neck, as well as being a prognostic marker for non-Hodgkin's lymphoma.
The variant isoforms of CD44 (CD44v) are highly expressed on epithelial cells but not leukocytes, and their association with cell transformation and cancer is also well established, supported by numerous immunohistochemical studies including those looking at HNSCC. Studies looking at the role of CD44v6 in Chrohns disease, in which clone VFF-18 was generated and characterised, revealed that VFF-18 binds to CD44v6 with high affinity when compared with anti-CD44v6 clone 2F10, and is suitable for detecting CD44v6 in inflammatory bowel disease (IBD) (Reinisch et a.l 1998).
Mouse anti Human CD44v6, clone VFF-18 is suitable for detecting CD44v6 in inflammatory bowel disease (IBD) (Reinisch et al, 1998).
Many functional properties of CD44s have been determined, including cell adhesion, lymphocyte activation, signal transduction, hematopoiesis, recirculation and homing, and CD44v6 has been particularly implicated in leukocyte to enothelial cell attachment and rolling, as well as homing to sites of inflammation and peripheral organs.
As well as its role as a receptor for hyaluronic acid (HA), CD44s also interacts with other ligands including matrix metalloproteinases(MMPs), fibronectin and collagens, and has been linked with cancer of the breast, colon, stomach and squamous cell carcinomas (HNSCC) of the head and neck, as well as being a prognostic marker for non-Hodgkin's lymphoma.
The variant isoforms of CD44 (CD44v) are highly expressed on epithelial cells but not leukocytes, and their association with cell transformation and cancer is also well established, supported by numerous immunohistochemical studies including those looking at HNSCC. Studies looking at the role of CD44v6 in Chrohns disease, in which clone VFF-18 was generated and characterised, revealed that VFF-18 binds to CD44v6 with high affinity when compared with anti-CD44v6 clone 2F10, and is suitable for detecting CD44v6 in inflammatory bowel disease (IBD) (Reinisch et a.l 1998).
Mouse anti Human CD44v6, clone VFF-18 is suitable for detecting CD44v6 in inflammatory bowel disease (IBD) (Reinisch et al, 1998).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
CD44 antigen isoform 1
NCBI Official Synonym Full Names
CD44 molecule (Indian blood group)
NCBI Official Symbol
CD44
NCBI Official Synonym Symbols
IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
NCBI Protein Information
CD44 antigen; GP90 lymphocyte homing/adhesion receptor; Hermes antigen; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; epican; extracellular matrix receptor III; hematopoietic cell E- and L-selectin ligand; heparan sulfate proteoglycan; homing function and Indian blood group system; hyaluronate receptor; phagocytic glycoprotein 1
UniProt Protein Name
CD44 antigen
UniProt Gene Name
CD44
UniProt Synonym Gene Names
LHR; MDU2; MDU3; MIC4; ECMR-III; PGP-1; PGP-I
UniProt Entry Name
CD44_HUMAN
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Product Notes
The CD44v6 cd44 (Catalog #AAA49818) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD44v6 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD44v6 cd44 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD44v6, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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