Mouse anti-Human CD45 Monoclonal Antibody | anti-CD45 antibody

CD45 Monoclonal Antibody

Cat. Num. AAA330115

• Gene Names: PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot
• Purity: Affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
• Synonyms: CD45, Antibody; CD45 Monoclonal Antibody; Mouse Anti-CD45 Monoclonal Antibody; PTPRC; CD45; Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; L-CA; T200; anti-CD45 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: The antibody detects endogenous CD45 proteins.
• Purity/Purification: Affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
• Form/Format: Liquid; PBS, pH 7.4, Containing 0.02% Sodium Azide As Preservative and 50% Glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1306

• Applicable Applications for anti-CD45 antibody: IHC (Immunohistochemistry), IF (Immunofluorescence), WB (Western Blot)
• Immunogen: Synthetic Peptide
• Preparation and Storage: Stable for one year at -20 degree C from date of shipment.; For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

IHC (Immunohistochemisry)

(Fig.3. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue. 1, CD45 Monoclonal Antibody was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)

IF (Immunofluorescence)

(Fig.2. Immunofluorescence analysis of human liver cancer tissue. 1, CD45 Monoclonal Antibody (red) was diluted at 1:200 (4 degree C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)

WB (Western Blot)

(Fig.1. Western blot analysis of Hela, diluted at 1:2000.)

Related Product Information for anti-CD45 antibody

Protein tyrosine phosphatase, receptor type C encoded by PTPRC is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of PTPRC, which encode distinct isoforms, have been reported.

NCBI and Uniprot Product Information

• NCBI GI #: 392307007
• NCBI GeneID: 5788
• NCBI Accession #: NP_002829.3
• NCBI GenBank Nucleotide #: NP_002829.3
• UniProt Accession #: P08575
• Molecular Weight: 147 KD
• NCBI Official Full Name: receptor-type tyrosine-protein phosphatase C isoform 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase receptor type C
• NCBI Official Symbol: PTPRC
• NCBI Official Synonym Symbols: LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
• NCBI Protein Information: receptor-type tyrosine-protein phosphatase C
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase C
• UniProt Gene Name: PTPRC
• UniProt Synonym Gene Names: CD45; L-CA
• UniProt Entry Name: PTPRC_HUMAN