IHC (Immunohiostchemistry)
(Fig: 2 Immunohistochemical analysis of CD45RA in human spleen using CD45RA antibody (Clone: 158-4D3) at 1: 500 dilution.)
Mouse anti-Human CD45RA Monoclonal Antibody | anti-CD45RA antibody
Monoclonal Antibody to CD45RA (Clone: 158-4D3)
Gene Names
PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry
Purity
PurifiedProtein G Chromatography
Synonyms
CD45RA, Antibody; Monoclonal Antibody to CD45RA (Clone: 158-4D3); PTPRC; CD45; Leukocyte common antigen; T200; anti-CD45RA antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a Kappa
Clone Number
158-4D3
Purity/Purification
Purified
Protein G Chromatography
Protein G Chromatography
Form/Format
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Sequence Length
1306
Applicable Applications for anti-CD45RA antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry)
Antigen
CD45RA
Immunogen Information
Stimulated human leukocytes were used as immunogen to generate the CD45RA antibody.
Conjugation
Unconjugated
Preparation and Storage
Store the antibody at 4 degree C; stable for 6 months.
For long-term storage; store at -20 degree C.
Avoid repeated freeze and thaw cycles.
For long-term storage; store at -20 degree C.
Avoid repeated freeze and thaw cycles.
IHC (Immunohiostchemistry)
(Fig: 2 Immunohistochemical analysis of CD45RA in human spleen using CD45RA antibody (Clone: 158-4D3) at 1: 500 dilution.)
IHC (Immunohiostchemistry)
(Fig: 2 Immunohistochemical analysis of CD45RA in human spleen using CD45RA antibody (Clone: 158-4D3) at 1: 500 dilution.)
FCM/FACS (Flow Cytometry)
(Fig. 1: Cell Surface flow analysis of hCD45RA in PBMC (Lymphocytes gated) using 1 ug antibody per 10^6 cells. Green represents isotype control; red represents anti-hCD45RA antibody (36-3014). Goat anti-mouse FITC conjugated secondary antibody was used.)
FCM/FACS (Flow Cytometry)
(Fig. 1: Cell Surface flow analysis of hCD45RA in PBMC (Lymphocytes gated) using 1 ug antibody per 10^6 cells. Green represents isotype control; red represents anti-hCD45RA antibody (36-3014). Goat anti-mouse FITC conjugated secondary antibody was used.)
Related Product Information for anti-CD45RA antibody
CD45 (leukocyte common antigen), the most common hematopoietic lineage marker, is a transmembrane glycoprotein tyrosine phosphatase with important roles in immune system signal transduction pathways. Antibody panels have been instrumental in identifying multiple isoforms, including CD45RA. Isoforms differ in their extracellular domain and are generated by alternative splicing of exons 4, 5 and 6 encoding peptide segments called A, B, and C, respectively. At least five different isoforms have been identified in humans: containing all three (ABC), two (AB and BC), one (B) or no (O) exons. A given antibody recognizes either every isoform (CD45 antibody) or only a subset (restricted CD45R antibody). The CD45RA 158-4D3 antibody clone reacts with the ABC and BC isoforms. CD45RA has a molecular weight of 205-220 kDa.
Product Categories/Family for anti-CD45RA antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
130,898 Da
NCBI Official Full Name
receptor-type tyrosine-protein phosphatase C isoform 1
NCBI Official Synonym Full Names
protein tyrosine phosphatase, receptor type C
NCBI Official Symbol
PTPRC
NCBI Official Synonym Symbols
LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
NCBI Protein Information
receptor-type tyrosine-protein phosphatase C
UniProt Protein Name
Receptor-type tyrosine-protein phosphatase C
UniProt Gene Name
PTPRC
UniProt Synonym Gene Names
CD45; L-CA
Similar Products
Product Notes
The CD45RA ptprc (Catalog #AAA78454) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to CD45RA (Clone: 158-4D3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD45RA can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CD45RA ptprc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD45RA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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