Mouse anti-Human CD45RA Monoclonal Antibody | anti-PTPRC antibody

CD45RA (Leucocyte Marker) Mouse Monoclonal Antibody

Cat. Num. AAA62550

• Gene Names: PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: CD45RA, Antibody; CD45RA (Leucocyte Marker) Mouse Monoclonal Antibody; B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein; anti-PTPRC antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: 158-4D3
• Specificity: Recognizes a protein of 205kDa-220kDa, identified as CD45RA [Workshop V; Code CD45.38]. CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 1306

• Applicable Applications for anti-PTPRC antibody: IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Cellular Localization: Cell Surface
• Immunogen: Stimulated human leukocytes
• Hu-Chromosome Location: 1q31.3
• Positive Control: Daudi, Raji, IM9 and human lymphocytes. Human lymph nodes and tonsils.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

FCM/FACS (Flow Cytometry)

(FACS Analysis human of PBMC using CD45RA Monoclonal Antibody (158-4D3) (red) and isotype control (green).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Tonsil stained with CD45RA Monoclonal Antibody (158-4D3).)

References

Oxford University Press, Oxford, p511-515, 1995. Clement LT. J Clin Immunol. 1992, 12(1):1-10. Yamada A, et al. Cell Immunol. 1992, 142(1):114-24. Mahalingam M, et al. Clin Immunol Immunopathol. 1996,81(2):210-214. Jacob MC, et al. Am J Hematol. 1992, 39(1):45-5

NCBI and Uniprot Product Information

• NCBI GI #: 392307007
• NCBI GeneID: 5788
• NCBI Accession #: NP_002829.3
• NCBI GenBank Nucleotide #: NM_002838.4
• UniProt Accession #: P08575; Q16614; Q9H0Y6; A8K7W6
• Molecular Weight: 205-220kDa
• NCBI Official Full Name: receptor-type tyrosine-protein phosphatase C isoform 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, receptor type C
• NCBI Official Symbol: PTPRC
• NCBI Official Synonym Symbols: LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
• NCBI Protein Information: receptor-type tyrosine-protein phosphatase C
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase C
• UniProt Gene Name: PTPRC
• UniProt Synonym Gene Names: CD45; L-CA
• UniProt Entry Name: PTPRC_HUMAN