CD45RA or A/B Recombinant Antibody | CD45RA recombinant antibody

Anti-CD45RA or A/B [OX-30], Rabbit IgG, kappa

Cat. Num. AAA72475

• Gene Names: Ptprc; Lca; RT7; CD45; L-CA; T200
• Reactivity: Rat
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Protein A affinity purified
• Synonyms: CD45RA or A/B, Recombinant Antibody; Anti-CD45RA or A/B [OX-30], Rabbit IgG, kappa; CD45RA or A/B; Anti-CD45RA or A/B [OX-30]; PTPRC; B220; CD45; CD45 R; GP180; L-CA; LCA; LY5; T200; protein tyrosine phosphatase; receptor type C; Receptor-type tyrosine-protein phosphatase C; CD45RA recombinant antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Monoclonal
• Isotype: Rabbit IgG kappa
• Clone Number: OX-30
• Specificity: This antibody recognizes all molecular forms of rat L-CA, and is noncompetitive with OX-1, 28 and 29 in binding to L-CA. While purified L-CA inhibited binding of this antibody to lymph node cells, no such inhibition of binding was seen with reduced and alkylated L-CA.
• Purity/Purification: Protein A affinity purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for CD45RA recombinant antibody: Blocking, IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry)
• Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

WB (Western Blot)

(Western Blotusinganti-CD45RAorA/B(Receptor-typetyrosine-proteinphosphataseC)antibodyOX-30(AAA72475) Ratthymuslysatesamples(35ugproteininRIPAbuffer)wereresolvedona10%SDSPAGEgelandblotsprobedwiththechimericrabbitversionofOX-30(AAA72475)at2ug/mlbeforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.TheexpectedbandsizeforunmodifiedCD45RAis~143.3kDa,butthisproteinisalsohighlyglycosylated(UniProtId:P04157).AAA72475successfullydetectedCD45RAorA/Binratthymustissuelysate.)

IF (Immunofluorescence)

(Immunofluorescencestainingoffixedratsplenocyteswithanti-CD45RAorA/B(Receptor-typetyrosine-proteinphosphataseC)antibodyOX-30(AAA72475) Immunofluorescenceanalysisofparaformaldehydefixedrat(Rattusnorvegicus)splenocytesonShi-fixcoverslips,permeabilizedwith0.15%TritonstainedwiththechimericrabbitversionofOX-30(AAA72475)at10ug/mlfor1hfollowedbyAlexaFluor 488secondaryantibody(1ug/ml),showingmembraneandcytoplasmicstainingofasubsetofcells.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72475,DAPI,mergedchannelsandanisotypecontrol.Theisotypecontrolwasstainedwithananti-FluoresceinantibodyfollowedbyAlexaFluor 488secondaryantibody.)

FCM/FACS (Flow Cytometry)

(Flow-cytometryusinganti-CD45RAorA/B(Receptor-typetyrosine-proteinphosphataseCisoform)antibodyOX-30(AAA72475) Stainingofrat(Rattusnorvegicus)lymphocyteswitheitheranisotypecontrol(A)ortherabbit-chimericversionofOX-30(B,AAA72475)ataconcentrationof1ug/mlfor30minsatRT.Afterwashing,boundantibodywasdetectedusinganAlexaFluor488conjugateddonkeyanti-rabbitantibody(ab150073)andcellsanalysedonaFACSCantoflow-cytometer.)

Related Product Information for CD45RA recombinant antibody

This antibody was prepared by immunizing BALB/c mice with rat lymph node glycoproteins.

References

Woollett et al. Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes. Eur J Immunol. 1985 Feb;15(2):168-73. N/A2578966
Note on publication:
Describes the original use of this antibody in FC and blocking analysis to assess the molecular and antigenic heterogenity of rat L-CA.

NCBI and Uniprot Product Information

• NCBI GI #: 158138490
• NCBI GeneID: 24699
• NCBI Accession #: NP_001103357.1
• NCBI GenBank Nucleotide #: NM_001109887.1
• UniProt Accession #: P04157
• Molecular Weight: 134,182 Da
• NCBI Official Full Name: receptor-type tyrosine-protein phosphatase C isoform 3
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, receptor type, C
• NCBI Official Symbol: Ptprc
• NCBI Official Synonym Symbols: Lca; RT7; CD45; L-CA; T200
• NCBI Protein Information: receptor-type tyrosine-protein phosphatase C; Protein tyrosine phosphatase, receptor-type, c polypeptide; T200 glycoprotein; leucocyte common antigen; leukocyte common antigen A; leukocyte common antigen B
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase C
• UniProt Gene Name: Ptprc
• UniProt Synonym Gene Names: L-CA
• UniProt Entry Name: PTPRC_RAT