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FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing JK cells stained with AAA278880 (red line) at 1:200. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
Mouse anti-Human, Mouse CD48 Monoclonal Antibody | anti-CD48 antibody
CD48 Monoclonal Antibody
Gene Names
CD48; BCM1; BLAST; hCD48; mCD48; BLAST1; SLAMF2; MEM-102
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, ELISA
Purity
>95%, Protein G purified
Synonyms
CD48, Antibody; CD48 Monoclonal Antibody; Antigen CD48 antibody; B cell membrane protein antibody; B lymphocyte activation marker BLAST 1 antibody; B-cell activation marker antibody; B-lymphocyte activation marker BLAST-1 antibody; BCM 1 surface antigen antibody; BCM1 antibody; BCM1 surface antigen antibody; BLAST 1 antibody; BLAST antibody; BLAST1 antibody; CD 48 antibody; CD48 antibody; CD48 antigen (B cell membrane protein) antibody; CD48 antigen antibody; CD48 molecule antibody; CD48 protein antibody; CD48_HUMAN antibody; hCD48 antibody; Leucocyte antigen MEM 102 antibody; Leukocyte antigen MEM-102 antibody; mCD48 antibody; MEM 102 antibody; MEM-102 antibody; MEM102 antibody; Signaling lymphocytic activation molecule 2 antibody; SLAM family member 2 antibody; SLAMF 2 antibody; SLAMF2 antibody; TCT.1 antibody; anti-CD48 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6F9H2
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH7.4
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH7.4
Applicable Applications for anti-CD48 antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), WB (Western Blot), ELISA
Immunogen Species
Human
Immunogen
Recombinant Human CD48 protein (27-220AA)
Conjugation
Non-conjugated
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing JK cells stained with AAA278880 (red line) at 1:200. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing JK cells stained with AAA278880 (red line) at 1:200. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
IF (Immunofluorescence)
(Immunofluorescence staining of JK cells with AAA278880 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of JK cells with AAA278880 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)
WB (Western Blot)
(Western BlotPositive WB detected in: 20ug Raji whole cell lysateCD48 antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000, 1:128000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 28, 20 KDaObserved band size: 43 KDaExposure time?5min)
WB (Western Blot)
(Western BlotPositive WB detected in: 20ug Raji whole cell lysateCD48 antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000, 1:128000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 28, 20 KDaObserved band size: 43 KDaExposure time?5min)
WB (Western Blot)
(Western BlotPositive WB detected in: Jurkat whole cell lysate, Raji whole cell lysate, Daudi whole cell lysate, Mouse liver tissueAll lanes: CD48 antibody at 1:4000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 28, 20KDaObserved band size: 43 KDaExposure time?5min)
WB (Western Blot)
(Western BlotPositive WB detected in: Jurkat whole cell lysate, Raji whole cell lysate, Daudi whole cell lysate, Mouse liver tissueAll lanes: CD48 antibody at 1:4000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 28, 20KDaObserved band size: 43 KDaExposure time?5min)
Product Categories/Family for anti-CD48 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
19,429 Da
NCBI Official Full Name
CD48 antigen
NCBI Official Synonym Full Names
CD48 molecule
NCBI Official Symbol
CD48
NCBI Official Synonym Symbols
BCM1; BLAST; hCD48; mCD48; BLAST1; SLAMF2; MEM-102
NCBI Protein Information
CD48 antigen
UniProt Protein Name
CD48 antigen
UniProt Gene Name
CD48
UniProt Synonym Gene Names
BCM1; BLAST1; SLAMF2
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Product Notes
The CD48 cd48 (Catalog #AAA278880) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD48 Monoclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD48 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the CD48 cd48 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD48, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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