Standard Curve (Sample)
(Representative Standard Curve using mouse monoclonal anti-CD59 (CM0111) for ELISA capture of human recombinant CD59 extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.)
Mouse anti-Human CD59 Monoclonal Antibody | anti-CD59 antibody
CD59 (glycoprotein) M011 Antibody
Gene Names
CD59; 1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20
Reactivity
Human
Applications
Immunoprecipitation, Immunocytochemistry, ELISA, Western Blot
Purity
Protein G chromatography
Synonyms
CD59, Antibody; CD59 (glycoprotein) M011 Antibody; CD59 (glycoprotein); CD059 M011; anti-CD59 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
M011
Specificity
Clone M011 detects 15-20kDa* bands corresponding to CD59 on SDS-PAGE immunoblots of native human MDA-MB-231, A549, A431, and MCF7 cell lysates, as well as recombinant human CD59 protein. The antibody does not detect denatured CD59. The antibody can be used for multiple applications including ELISA, western blot, immunocytochemical labeling and immunoprecipitation. In addition, the antibody labels live, unfixed A549 and MeWo cells.
Purity/Purification
Protein G chromatography
Form/Format
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-CD59 antibody
IP (Immunoprecipitation), ICC (Immunocytochemistry), ELISA, WB (Western Blot)
Immunogen
Clone M011 was generated from a proprietary antigen related to the mature form of human CD59 from the MDA-MB-231 breast carcinoma cell line.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Standard Curve (Sample)
(Representative Standard Curve using mouse monoclonal anti-CD59 (CM0111) for ELISA capture of human recombinant CD59 extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.)
Standard Curve (Sample)
(Representative Standard Curve using mouse monoclonal anti-CD59 (CM0111) for ELISA capture of human recombinant CD59 extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.)
ICC (Immunocytochemistry)
(Immunocytochemical labeling of CD59 in paraformaldehyde fixed human A549 cells. The cells were labeled with mouse monoclonal anti-CD59 (clone M011). The antibody was detected using goat anti-mouse Ig DyLight 594.)
ICC (Immunocytochemistry)
(Immunocytochemical labeling of CD59 in paraformaldehyde fixed human A549 cells. The cells were labeled with mouse monoclonal anti-CD59 (clone M011). The antibody was detected using goat anti-mouse Ig DyLight 594.)
WB (Western Blot)
(Western blot analysis of recombinant human CD59 protein (lanes 1 & 5), A431 (lanes 2 & 6), A549 (lanes 3 & 7), and MCF7 (lanes 4 & 8) whole cell lysates under native conditions. The blots were probed with mouse monoclonal anti-CD59 (CM0111) at 1:1000 (lanes 1-4) and anti-CD59 (CM0151) at 1:1000 (lanes 5-8).)
WB (Western Blot)
(Western blot analysis of recombinant human CD59 protein (lanes 1 & 5), A431 (lanes 2 & 6), A549 (lanes 3 & 7), and MCF7 (lanes 4 & 8) whole cell lysates under native conditions. The blots were probed with mouse monoclonal anti-CD59 (CM0111) at 1:1000 (lanes 1-4) and anti-CD59 (CM0151) at 1:1000 (lanes 5-8).)
Related Product Information for anti-CD59 antibody
CD59 is a GPI-anchored membrane protein that is an inhibitor of the complement membrane attack complex (MAC). CD59 binds to complement components C8 and C9, preventing C9 polymerization and insertion into membranes. Rare cases of CD59 deficiency have been reported to cause paroxysmal nocturnal hemoglobinuria in human patients. Expression of CD59 on tumor cells and viral infected cells makes them resist antibody-dependent complement-mediated lysis. Inhibitors of CD59 expression or activity may suppress tumor cell resistance to complement-mediated attack, and these technologies have been actively pursued for therapeutic applications. In addition, CD59 may regulate insulin secretion by modulating exocytosis, and a glycated form of CD59 with no MAC inhibitory activity is found in diabetic patients.
References
Mevorach, D (2015) Mol Immunol. 67:51.
Ghosh P et al. (2015) Endocr Rev. 36(3):272.
Sugita, Y & Masuho, Y (1995) Immunotechnology 1:157.
Ghosh P et al. (2015) Endocr Rev. 36(3):272.
Sugita, Y & Masuho, Y (1995) Immunotechnology 1:157.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
14,177 Da
NCBI Official Full Name
CD59 glycoprotein preproprotein
NCBI Official Synonym Full Names
CD59 molecule, complement regulatory protein
NCBI Official Symbol
CD59
NCBI Official Synonym Symbols
1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20
NCBI Protein Information
CD59 glycoprotein; protectin; 1F5 antigen; MEM43 antigen; Ly-6-like protein; T cell-activating protein; human leukocyte antigen MIC11; lymphocytic antigen CD59/MEM43; 20 kDa homologous restriction factor; membrane inhibitor of reactive lysis; membrane att
UniProt Protein Name
CD59 glycoprotein
UniProt Gene Name
CD59
UniProt Synonym Gene Names
MIC11; MIN1; MIN2; MIN3; MSK21; HRF-20; HRF20; MAC-IP; MACIF; MIRL
UniProt Entry Name
CD59_HUMAN
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Product Notes
The CD59 cd59 (Catalog #AAA71577) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD59 (glycoprotein) M011 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD59 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ICC (Immunocytochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CD59 cd59 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD59, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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