IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human Tonsil stained with CD59 Monoclonal Antibody (MACIF/629))
Mouse CD59 / Complement Regulatory Protein / Protectin Monoclonal Antibody | anti-CD59 antibody
CD59 / Complement Regulatory Protein / Protectin Mouse Monoclonal Antibody
Gene Names
CD59; 1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20
Reactivity
Human.Others not tested
Applications
Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, Functional Assay
Synonyms
CD59 / Complement Regulatory Protein / Protectin, Antibody; CD59 / Complement Regulatory Protein / Protectin Mouse Monoclonal Antibody; 20kDa homologous restriction factor (HRF20); Complement regulatory protein; Human leukocyte antigen MIC11; MAC-inhibitory protein (MACIP); Membrane attack complex inhibition factor (MACIF); Membrane inhibitor of reactive lysis; MIRL; MSK21; Protectin; T cell activating protein; anti-CD59 antibody
Host
Mouse
Reactivity
Human.
Others not tested
Others not tested
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
MACIF/629
Specificity
Reacts with human CD59, a 20kDa glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein. CD59 regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. It inhibits formation of MAC, thus protecting cells from complement-mediated lysis. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH). This MAb is useful for study on GPI-anchored proteins, PNH and CD59 functions. CD59 is widely distributed on cells in all tissues. The expression of CD59 on erythrocytes is important for their survival.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
128
Applicable Applications for anti-CD59 antibody
IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), FA (Functional Assay)
Cellular Localization
Cell Surface
Immunogen
Recombinant full-length human CD59 protein
Hu-Chromosome Location
11p13
Positive Control
Daudi, CEM, K562, HPB.ALL, Jurkat, Raji, and human lymphocytes. Tonsil, Cervix or Bladder.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human Tonsil stained with CD59 Monoclonal Antibody (MACIF/629))
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human Tonsil stained with CD59 Monoclonal Antibody (MACIF/629))
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Tongue stained with CD59 Monoclonal Antibody (MACIF/629))
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Tongue stained with CD59 Monoclonal Antibody (MACIF/629))
References
A E Fritzinger, F. Marciano-Cabral, et al. (2006) Infection and Immunity 74(2):1189-1195. J Zhang, C Gerhardinger, M Lorenzi (2002) Diabetes 51(12): 3499-3504
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
20kDa
NCBI Official Full Name
CD59 glycoprotein preproprotein
NCBI Official Synonym Full Names
CD59 molecule
NCBI Official Symbol
CD59
NCBI Official Synonym Symbols
1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20
NCBI Protein Information
CD59 glycoprotein
UniProt Protein Name
CD59 glycoprotein
UniProt Gene Name
CD59
UniProt Synonym Gene Names
MIC11; MIN1; MIN2; MIN3; MSK21; HRF-20; HRF20; MAC-IP; MACIF; MIRL
UniProt Entry Name
CD59_HUMAN
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Product Notes
The CD59 cd59 (Catalog #AAA62872) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD59 / Complement Regulatory Protein / Protectin Mouse Monoclonal Antibody reacts with Human. Others not tested and may cross-react with other species as described in the data sheet. AAA Biotech's CD59 / Complement Regulatory Protein / Protectin can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), FA (Functional Assay). Researchers should empirically determine the suitability of the CD59 cd59 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD59 / Complement Regulatory Protein / Protectin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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