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FCM (Flow Cytometry)
(Staining of thrombin activated platelets with Mouse anti Human CD62P)
Mouse CD62P Monoclonal Antibody | anti-CD62P antibody
MOUSE ANTI HUMAN CD62P
Gene Names
SELP; CD62; GRMP; PSEL; CD62P; GMP140; LECAM3; PADGEM
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Synonyms
CD62P, Antibody; MOUSE ANTI HUMAN CD62P; P-SELECTIN; GMRP; GRMP; anti-CD62P antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
AK-6
Specificity
Mouse anti Human CD62P antibody, clone AK-6 recognizes the CD62P, also known as P-selectin, Granule membrane protein 140, GMP140, Leukocyte-endothelial cell adhesion molecule 3 or Platelet activation dependent granule-external membrane protein. CD62P is a 830 amino acid, including a 41 amino acid signal peptide, ~140 kDa single pass type I transmembrane glycoprotein expressed on activated platelets and endothelial cell CD62P plays an important role in adhesive processes between leucocytes and endothelial cells. CD62P is a component of the platelet alpha granule and is rapidly translocated to the plasma membrane upon activation
Form/Format
Purified IgG - liquid; Phosphate buffered saline
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
830
Applicable Applications for anti-CD62P antibody
Immunohistology Frozen, Flow cytometry (FC), Immunofluorescence (IF), Immunohistology Paraffin, Western Blot (WB)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Immunohistology: This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections but staining may be enhanced using Sodium Citrate buffer pH6.0
Immunohistology - Frozen: Minimum Dilution: 1/1000; Maximum Dilution: 1/5000
Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/100
Immunohistology - Paraffin: Minimum Dilution: 1/500; Maximum Dilution: 1/2000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
(1)Staining may be enhanced using Sodium Citrate buffer pH6.0
Immunohistology: This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections but staining may be enhanced using Sodium Citrate buffer pH6.0
Immunohistology - Frozen: Minimum Dilution: 1/1000; Maximum Dilution: 1/5000
Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/100
Immunohistology - Paraffin: Minimum Dilution: 1/500; Maximum Dilution: 1/2000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
(1)Staining may be enhanced using Sodium Citrate buffer pH6.0
RRID
AB_1125272
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Immunogen
Human plateletmembrane glycoproteins.
Histology Positive Control Tissue
Tonsil
Target Species
Human
Species Cross-Reactivity
Reacts with: Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species. Cross reactivity is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators.
N.B. Antibody reactivity and working conditions may vary between species. Cross reactivity is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators.
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
FCM (Flow Cytometry)
(Staining of thrombin activated platelets with Mouse anti Human CD62P)
FCM (Flow Cytometry)
(Staining of thrombin activated platelets with Mouse anti Human CD62P)
FCM (Flow Cytometry)
(Staining of thrombin activated human platelets with Mouse anti Human CD62P:Azide Free)
FCM (Flow Cytometry)
(Staining of thrombin activated human platelets with Mouse anti Human CD62P:Azide Free)
FCM (Flow Cytometry)
(Staining of thrombin activated human peripheral blood platelets with Mouse anti Human CD62P: FITC)
FCM (Flow Cytometry)
(Staining of thrombin activated human peripheral blood platelets with Mouse anti Human CD62P: FITC)
FCM (Flow Cytometry)
(Staining of human peripheral blood platelets with Mouse anti Human CD62P (AAA12247))
FCM (Flow Cytometry)
(Staining of human peripheral blood platelets with Mouse anti Human CD62P (AAA12247))
IHC (Immunohistochemistry)
(Staining of human tonsil showing capillary endothelium. Formalin fixed, paraffin processed tissue with Mouse anti Human CD62P (P-Selectin) (AAA12247))
IHC (Immunohistochemistry)
(Staining of human tonsil showing capillary endothelium. Formalin fixed, paraffin processed tissue with Mouse anti Human CD62P (P-Selectin) (AAA12247))
Related Product Information for anti-CD62P antibody
Mouse anti Human CD62P antibody, clone AK-6 recognizes the CD62P glycoprotein, a 140kD molecule expressed by activated platelets and endothelial cells. CD62P is also known as P-selectin, PADGEM or GMP140 and plays an important role in adhesive processes between leucocytes and endothelial cells. CD62P is a component of the alpha granule and is translocated to the plasma membrane upon activation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
P-selectin
NCBI Official Synonym Full Names
selectin P (granule membrane protein 140kDa, antigen CD62)
NCBI Official Symbol
SELP
NCBI Official Synonym Symbols
CD62; GRMP; PSEL; CD62P; GMP140; LECAM3; PADGEM
NCBI Protein Information
P-selectin; CD62 antigen-like family member P; GMP-140; granule membrane protein 140; granulocyte membrane protein; leukocyte-endothelial cell adhesion molecule 3; platelet activation dependent granule-external membrane protein; platelet alpha-granule membrane protein
UniProt Protein Name
P-selectin
UniProt Gene Name
SELP
UniProt Synonym Gene Names
GMRP; GRMP; GMP-140; LECAM3; PADGEM
UniProt Entry Name
LYAM3_HUMAN
Similar Products
Product Notes
The CD62P selp (Catalog #AAA12247) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD62P can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC), Immunofluorescence (IF), Immunohistology Paraffin, Western Blot (WB). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Immunohistology: This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections but staining may be enhanced using Sodium Citrate buffer pH6.0 Immunohistology - Frozen: Minimum Dilution: 1/1000; Maximum Dilution: 1/5000 Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/100 Immunohistology - Paraffin: Minimum Dilution: 1/500; Maximum Dilution: 1/2000 Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. (1)Staining may be enhanced using Sodium Citrate buffer pH6.0. Researchers should empirically determine the suitability of the CD62P selp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD62P, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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