IP (Immunoprecipitation)
(CD69 was immunoprecipitated using:Lane A:0.5 mg HL-60 Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell Lysate0.5 uL anti-CD69 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CD69 rabbit monoclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 23 kDaObserved band size: 23 kDa)
Rabbit anti-Human CD69 Monoclonal Antibody | anti-CD69 antibody
Recombinant Anti-CD69 Antibody, Rabbit Monoclonal
Gene Names
CD69; AIM; EA1; MLR-3; CLEC2C; GP32/28; BL-AC/P26
Reactivity
Human
Applications
Immunoprecipitation, ELISA, Western Blot
Purity
Protein A
Synonyms
CD69, Antibody; Recombinant Anti-CD69 Antibody, Rabbit Monoclonal; CD69 Antibody, Rabbit MAb; CD69 molecule; Anti-AIM Antibody; Anti-BL-AC/P26 Antibody; Anti-CLEC2C Antibody; Anti-EA1 Antibody; Anti-GP32/28 Antibody; Anti-MLR-3 Antibody; anti-CD69 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
074
Specificity
Human CD69
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CD69 antibody
IP (Immunoprecipitation), ELISA, WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Human CD69 Protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human CD69 (rh CD69).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(CD69 was immunoprecipitated using:Lane A:0.5 mg HL-60 Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell Lysate0.5 uL anti-CD69 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CD69 rabbit monoclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 23 kDaObserved band size: 23 kDa)
IP (Immunoprecipitation)
(CD69 was immunoprecipitated using:Lane A:0.5 mg HL-60 Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell Lysate0.5 uL anti-CD69 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CD69 rabbit monoclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 23 kDaObserved band size: 23 kDa)
WB (Western Blot)
(Anti-CD69 rabbit monoclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: HL60 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:23 kDaObserved band size:23 kDa)
WB (Western Blot)
(Anti-CD69 rabbit monoclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: HL60 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:23 kDaObserved band size:23 kDa)
Related Product Information for anti-CD69 antibody
Early activation antigen CD69, also known as activation inducer molecule (AIM), is a single-pass type II membrane protein. Recently, cDNA clones encoding human and mouse CD69 were isolated and showed CD69 to be a member of the C-type lectin superfamily. It is one of the earliest cell surface antigens expressed by T cells following activation. Once expressed, CD69 acts as a costimulatory molecule for T cell activation and proliferation. In addition to mature T cells, CD69 is inducibly expressed by immature thymocytes, B cells, natural killer (NK) cells, monocytes, neutrophils and eosinophils, and is constitutively expressed by mature thymocytes and platelets. CD69 is involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets. The structure, chromosomal localization, expression and function of CD69 suggest that it is likely a pleiotropic immune regulator, potentially important in the activation and differentiation of a wide variety of hematopoietic cells. This membrane molecule transiently expresses on activated lymphocytes, and its selective expression in inflammatory infiltrates suggests that it plays a role in the pathogenesis of inflammatory diseases. CD69 plays a crucial role in the pathogenesis of allergen-induced eosinophilic airway inflammation and hyperresponsiveness and that CD69 could be a possible therapeutic target for asthmatic patients.
References
Ziegler SF, et al. (1994) The activation antigen CD69. Stem Cells. 12(5): 456-65.Marzio R, et al. (1999) CD69 and regulation of the immune function. Immunopharmacol Immunotoxicol. 21(3): 565-82.Lamana A, et al. (2006) The role of CD69 in acute neutrophil-mediated inflammation. Eur J Immunol. 36(10): 2632-8.Miki-Hosokawa T, et al. (2009) CD69 controls the pathogenesis of allergic airway inflammation. J Immunol. 183(12): 8203-15.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22,559 Da
NCBI Official Full Name
early activation antigen CD69
NCBI Official Synonym Full Names
CD69 molecule
NCBI Official Symbol
CD69
NCBI Official Synonym Symbols
AIM; EA1; MLR-3; CLEC2C; GP32/28; BL-AC/P26
NCBI Protein Information
early activation antigen CD69
UniProt Protein Name
Early activation antigen CD69
UniProt Gene Name
CD69
UniProt Synonym Gene Names
CLEC2C; AIM
UniProt Entry Name
CD69_HUMAN
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Product Notes
The CD69 cd69 (Catalog #AAA255333) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-CD69 Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD69 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CD69 cd69 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD69, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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