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WB (Western Blot)
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CD79A recombinant antibody

Rabbit anti-CD79A Recombinant Monoclonal Antibody [BLR234K]

Gene Names
CD79A; IGA; MB-1
Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
Purified
Synonyms
CD79A, Recombinant Antibody; Rabbit anti-CD79A Recombinant Monoclonal Antibody [BLR234K]; membrane-bound immunoglobulin-associated protein; surface IgM-associated protein; MB-1 membrane glycoprotein; MB-1; ig-alpha; CD79a molecule, immunoglobulin-associated alpha; CD79a antigen (immunoglobulin-associated alpha); CD antigen CD79a; B-cell antigen receptor complex-associated protein alpha chain; IGA; CD79a molecule; CD79A recombinant antibody
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For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
BLR234K
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
500ug/ml (varies by lot)
Applicable Applications for CD79A recombinant antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
IP: 12 ul/mg lysate
IHC: 1:50 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
ICC: 1:50 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 ul per 1 x 10^6 cells.
Immunogen
Between 176 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of mouse CD79A by western blot. Samples: Whole cell lysate (50 ug) from WEHI-231 (25 ug), NIH 3T3, CH27, TCMK-1, and A20 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD79A recombinant monoclonal antibody (AAA23878 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

WB (Western Blot)

(Detection of human CD79A by western blot. Samples: Whole cell lysate (50 ug) from Daudi, HeLa, Raji, HEK293T, and Ramos cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD79A recombinant monoclonal antibody (AAA23878 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

IP (Immunoprecipitation)

(Detection of human CD79A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Ramos cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD79A recombinant monoclonal antibody (AAA23878 lot 1) used for IP at 20 ul/mg lysate. CD79A was also immunoprecipitated by a second antibody against a different epitope of CD79A . For blotting immunoprecipitated CD79A, AAA23878 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.)

IHC (Immunohistchemistry)

(Detection of mouse CD79A by immunohistochemistry. Sample: FFPE section of mouse spleen. Antibody: Rabbit anti-CD79A recombinant monoclonal antibody (AAA23878-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

IHC (Immunohistochemistry)

(Detection of human CD79A by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-CD79A recombinant monoclonal antibody (AAA23878-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry)

(Detection of human CD79A by immunocytochemistry. Sample: FFPE section of Daudi cells. Antibody: Rabbit anti-CD79A recombinant monoclonal antibody (AAA23878-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry)

(Detection of mouse CD79A by immunocytochemistry. Sample: FFPE section of WEHI-231 cells. Antibody: Rabbit anti-CD79A recombinant monoclonal antibody (AAA23878-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry)

(Detection of human CD79A (shaded) in Daudi cells by flow cytometry. Antibody: Rabbit anti-CD79A recombinant monoclonal antibody (AAA23878) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry)

(Detection of mouse CD79A (shaded) in A20 cells by flow cytometry. Antibody: Rabbit anti-CD79A recombinant monoclonal antibody (AAA23878) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

Related Product Information for CD79A recombinant antibody
CD79A is required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79A is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P11912].
Product Categories/Family for CD79A recombinant antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
973
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
20,786 Da
NCBI Official Full Name
B-cell antigen receptor complex-associated protein alpha chain isoform 2
NCBI Official Synonym Full Names
CD79a molecule, immunoglobulin-associated alpha
NCBI Official Symbol
CD79A
NCBI Official Synonym Symbols
IGA; MB-1
NCBI Protein Information
B-cell antigen receptor complex-associated protein alpha chain; ig-alpha; MB-1 membrane glycoprotein; surface IgM-associated protein; CD79a antigen (immunoglobulin-associated alpha); membrane-bound immunoglobulin-associated protein
UniProt Protein Name
B-cell antigen receptor complex-associated protein alpha chain
UniProt Gene Name
CD79A
UniProt Synonym Gene Names
IGA; MB1
UniProt Entry Name
CD79A_HUMAN

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Product Notes

The CD79A cd79a (Catalog #AAA23878) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CD79A Recombinant Monoclonal Antibody [BLR234K] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD79A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). WB: 1:1000 IP: 12 ul/mg lysate IHC: 1:50 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. ICC: 1:50 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the CD79A cd79a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD79A, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.