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product-image-AAA213505_IHC11.jpg IHC (Immunohistochemisry) (Detection of human CD8 alpha by immunhistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse monoclonal anti-CD8 alpha antibody [C8/144B] (AAA213505 lot 1) used at 1:100. Secondary: DyLight 594-conjugated goat anti-mouse IgG .)

Mouse anti-Human CD8 alpha Monoclonal Antibody | anti-CD8alpha antibody

Mouse anti-CD8 alpha Monoclonal Antibody, Purified [C8/144B]

Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunohistochemistry
Purity
Purified
Synonyms
CD8 alpha, Antibody; Mouse anti-CD8 alpha Monoclonal Antibody, Purified [C8/144B]; Leu2 T-lymphocyte antigen; T-lymphocyte differentiation antigen T8/Leu-2; T-cell surface glycoprotein CD8 alpha chain; T-cell antigen Leu2; T8 T-cell antigen; T cell co-receptor; OKT8 T-cell antigen; Leu2; CD8 antigen, alpha polypeptide (p32); CD8; CD antigen CD8a; p32; CD8 subunit alpha; anti-CD8alpha antibody
Ordering
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Clone Number
C8/144B
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
250ug/ml (varies by lot)
Applicable Applications for anti-CD8alpha antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IHC (Immunohistochemistry)
Immunogen
A 13 amino acid synthetic peptide from the C-terminal cytoplasmic domain of alpha chain of human CD8 molecule
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

IHC (Immunohistochemisry)

(Detection of human CD8 alpha by immunhistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse monoclonal anti-CD8 alpha antibody [C8/144B] (AAA213505 lot 1) used at 1:100. Secondary: DyLight 594-conjugated goat anti-mouse IgG .)

product-image-AAA213505_IHC11.jpg IHC (Immunohistochemisry) (Detection of human CD8 alpha by immunhistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse monoclonal anti-CD8 alpha antibody [C8/144B] (AAA213505 lot 1) used at 1:100. Secondary: DyLight 594-conjugated goat anti-mouse IgG .)

IHC (Immunohiostchemistry)

(Detection of human CD8a by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Mouse anti-CD8a monoclonal antibody [C8/144B] (AAA213505-3). Secondary: HRP-conjugated goat anti-mouse IgG .)

product-image-AAA213505_IHC13.jpg IHC (Immunohiostchemistry) (Detection of human CD8a by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Mouse anti-CD8a monoclonal antibody [C8/144B] (AAA213505-3). Secondary: HRP-conjugated goat anti-mouse IgG .)

FCM/FACS (Flow Cytometry)

(Detection of human CD8 alpha (left) in human whole blood by flow cytometry. Antibody: Mouse anti-CD8 alpha monoclonal antibody [C8/144B] (AAA213505) or isotype control (right). Secondary: DyLight 488-conjugated goat anti-mouse IgG . Analysis performed on lymphocyte gated population.)

product-image-AAA213505_FCM15.jpg FCM/FACS (Flow Cytometry) (Detection of human CD8 alpha (left) in human whole blood by flow cytometry. Antibody: Mouse anti-CD8 alpha monoclonal antibody [C8/144B] (AAA213505) or isotype control (right). Secondary: DyLight 488-conjugated goat anti-mouse IgG . Analysis performed on lymphocyte gated population.)
Related Product Information for anti-CD8alpha antibody
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain [taken from NCBI Entrez Gene (Gene ID: 925)].
Product Categories/Family for anti-CD8alpha antibody

NCBI and Uniprot Product Information

NCBI GeneID
925
UniProt Accession #
UniProt Protein Name
T-cell surface glycoprotein CD8 alpha chain
UniProt Gene Name
CD8A
UniProt Synonym Gene Names
MAL
UniProt Entry Name
CD8A_HUMAN

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Product Notes

The CD8alpha cd8a (Catalog #AAA213505) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti-CD8 alpha Monoclonal Antibody, Purified [C8/144B] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD8 alpha can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CD8alpha cd8a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD8 alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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