SDS-PAGE
(SDS-PAGE Analysis Purified CD86 Mouse Monoclonal Antibody (C86/3713). Confirmation of Purity and Integrity of Antibody.)
Mouse anti-Human CD86 Monoclonal Antibody | anti-CD86 antibody
CD86 (Dendritic Cells Maturation Marker)
Gene Names
CD86; B70; B7-2; B7.2; LAB72; CD28LG2
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
CD86, Antibody; CD86 (Dendritic Cells Maturation Marker); Activation B7-2 antigen; B lymphocyte activation antigen B72; CD28 antigen ligand 2; CD28LG2; CLS1; CTLA-4 counter-receptor B7.2; Early T-cell co-stimulatory molecule 1; ETC-1; FUN-1; LAB72; Ly-58; MB7-2; TS/A-2; anti-CD86 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Clone Number
C86/3713
Specificity
Recognizes a protein of 70kDa, which is identified as CD86. CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkins disease.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Applicable Applications for anti-CD86 antibody
WB (Western Blot), IHC (Immunohistochemistry)
Immunogen
Recombinant fragment (around aa 66-195) of Human CD86 protein (exact sequence is proprietary)
Cellular Localization
Cell Surface
Hu-chromosome Location
3q13.33
Positive Control
293T or Jurkat cells. Monocytes and dendritic cells, activated T, B and natural killer cells in Lymph node or Tonsil.
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
SDS-PAGE
(SDS-PAGE Analysis Purified CD86 Mouse Monoclonal Antibody (C86/3713). Confirmation of Purity and Integrity of Antibody.)
SDS-PAGE
(SDS-PAGE Analysis Purified CD86 Mouse Monoclonal Antibody (C86/3713). Confirmation of Purity and Integrity of Antibody.)
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human tonsil stained with CD86 Mouse Monoclonal Antibody (C86/3713).)
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human tonsil stained with CD86 Mouse Monoclonal Antibody (C86/3713).)
WB (Western Blot)
(Western Blot Analysis of Ramos cell lysate using CD86 Mouse Monoclonal Antibody (C86/3713).)
WB (Western Blot)
(Western Blot Analysis of Ramos cell lysate using CD86 Mouse Monoclonal Antibody (C86/3713).)
References
Engel P et. al. Blood. 1994;84(5):1402-7.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The CD86 (Catalog #AAA215435) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD86 (Dendritic Cells Maturation Marker) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD86 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CD86 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD86, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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