FCM/FACS (Flow Cytometry)
(Flow-cytometry using anti-human UPAR antibody. HUCCT cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human UPAR antibody monoclonal antibody (#AAA120763, Green Histogram) at a concentration of 5ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.)
Mouse anti-Human CD87/PLAUR/uPAR Monoclonal Antibody | anti-CD87/PLAUR/uPAR antibody
Anti-Human CD87/PLAUR/uPAR Monoclonal Antibody (1A451)
Gene Names
PLAUR; CD87; UPAR; URKR; U-PAR
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Functional Assay, ELISA
Purity
>95% as determined by SDS-PAGE.Protein A/G purified from cell culture supernatant.
Synonyms
CD87/PLAUR/uPAR, Antibody; Anti-Human CD87/PLAUR/uPAR Monoclonal Antibody (1A451); MO3; Urokinase plasminogen activator surface receptor; uPAR; UPAR; CD87; PLAUR; U-PAR; Monocyte activation antigen Mo3; anti-CD87/PLAUR/uPAR antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
1A451
Purity/Purification
>95% as determined by SDS-PAGE.
Protein A/G purified from cell culture supernatant.
Protein A/G purified from cell culture supernatant.
Form/Format
Liquid; 0.01M PBS, pH 7.4.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD87/PLAUR/uPAR antibody
WB (Western Blot), FCM/FACS (Flow Cytometry), ELISA
Immunogen
Recombinant Human CD87/PLAUR/uPAR (Leu23-Arg303).
Preparation and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4 degree C short term (1-2 weeks). Store at -20 degree C 12 months. Store at -80 degree C long term.
FCM/FACS (Flow Cytometry)
(Flow-cytometry using anti-human UPAR antibody. HUCCT cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human UPAR antibody monoclonal antibody (#AAA120763, Green Histogram) at a concentration of 5ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.)
FCM/FACS (Flow Cytometry)
(Flow-cytometry using anti-human UPAR antibody. HUCCT cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human UPAR antibody monoclonal antibody (#AAA120763, Green Histogram) at a concentration of 5ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.)
WB (Western Blot)
(Various lysates were subjected to SDS PAGE followed by western blot with CD87 antibody (AAA120763) at 1ug/ml.Lane 1: HUCCT cell lysateLane 2: U937 cell lysateLane 3: U251 cell lysateLane 4: A549 cell lysateLane 5: Hela cell lysateLane 6: HUVEC cell lysate Lane 7: Mouse brain lysateLane 8: Mouse kidney lysateLane 9: Mouse lung lysateLane 10: Rat brain lysateLane 11: Rat kidney lysateLane 12: Rat lung lysateSecond Ab: Goat Anti-Mouse IgG H&L Polyclonal antibody, HRP (PMB96431) at 0.1 ug/mL.Predict MW: 37 kDa)
WB (Western Blot)
(Various lysates were subjected to SDS PAGE followed by western blot with CD87 antibody (AAA120763) at 1ug/ml.Lane 1: HUCCT cell lysateLane 2: U937 cell lysateLane 3: U251 cell lysateLane 4: A549 cell lysateLane 5: Hela cell lysateLane 6: HUVEC cell lysate Lane 7: Mouse brain lysateLane 8: Mouse kidney lysateLane 9: Mouse lung lysateLane 10: Rat brain lysateLane 11: Rat kidney lysateLane 12: Rat lung lysateSecond Ab: Goat Anti-Mouse IgG H&L Polyclonal antibody, HRP (PMB96431) at 0.1 ug/mL.Predict MW: 37 kDa)
Product Categories/Family for anti-CD87/PLAUR/uPAR antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36,978 Da
NCBI Official Full Name
urokinase plasminogen activator surface receptor isoform 1
NCBI Official Synonym Full Names
plasminogen activator, urokinase receptor
NCBI Official Symbol
PLAUR
NCBI Official Synonym Symbols
CD87; UPAR; URKR; U-PAR
NCBI Protein Information
urokinase plasminogen activator surface receptor; monocyte activation antigen Mo3; u-plasminogen activator receptor form 2; urokinase-type plasminogen activator (uPA) receptor
UniProt Protein Name
Urokinase plasminogen activator surface receptor
UniProt Gene Name
PLAUR
UniProt Synonym Gene Names
MO3; UPAR; U-PAR; uPAR
UniProt Entry Name
UPAR_HUMAN
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Product Notes
The CD87/PLAUR/uPAR plaur (Catalog #AAA120763) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Human CD87/PLAUR/uPAR Monoclonal Antibody (1A451) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD87/PLAUR/uPAR can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the CD87/PLAUR/uPAR plaur for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD87/PLAUR/uPAR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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