Mouse anti-Rat CD8a Monoclonal Antibody | anti-CD8a antibody

Anti-Rt CD8a Purified Low Endotoxin

Cat. Num. AAA129127

• Reactivity: Rat
• Applications: Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by protein-A affinity chromatography
• Synonyms: CD8a, Antibody; Anti-Rt CD8a Purified Low Endotoxin; Ly-2; Ly-B; Ly-35; Lyt-2; CD8a molecule; anti-CD8a antibody

• Host: Mouse
• Reactivity: Rat
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone Number: OX-8
• Specificity: The mouse monoclonal antibody OX-8 recognizes the hinge-like membrane-proximal extracellular domain of rat CD8a (32-34kDa; alpha chain of the CD8 antigen).
• Purity/Purification: Purified by protein-A affinity chromatography
• Form/Format: Purified Low Endotoxin. Low endotoxin azide free phosphate buffered saline (PBS) solution, 0.2um filter sterilized. Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD8a antibody: IHC (Immunohistochemistry), IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), FA (Functional Assay), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: High Mw glycoproteins from rat thymocytes
• Preparation and Storage: Store at 2-8 degree C. Do not freeze. Do not use after expiration date stamped on the label.

Related Product Information for anti-CD8a antibody

The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector Functional Application (FUNC)tion. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the Functional Application (FUNC)tional diversity of the TCR signaling.

References

*Pino SC, O'Sullivan-Murphy B, Lidstone EA, Yang C, Lipson KL, Jurczyk A, diIorio P, Brehm MA, Mordes JP, Greiner DL, Rossini AA, Bortell R: CHOP mediates endoplasmic reticulum stress-induced apoptosis in Gimap5-deficient T cells. PLoS One. 2009;4(5):e5468., URL: http://www.ncbi.nlm.nih.gov/pubmed/19424493,*Katsumata Y, Harigai M, Sugiura T, Kawamoto M, Kawaguchi Y, Matsumoto Y, Kohyama K, Soejima M, Kamatani N, Hara M: Attenuation of experimental autoimmune myositis by blocking ICOS-ICOS ligand interaction. J Immunol. 2007 Sep 15;179(6):3772-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/17785814,*Abe Y, Urakami H, Ostanin D, Zibari G, Hayashida T, Kitagawa Y, Grisham MB: Induction of Foxp3-expressing regulatory T-cells by donor blood transfusion is required for tolerance to rat liver allografts. PLoS One. 2009 Nov 23;4(11):e7840., URL: https://www.ncbi.nlm.nih.gov/pubmed/19956764,*Ishida S, Usui T, Yamashiro K, Kaji Y, Amano S, Ogura Y, Hida T, Oguchi Y, Ambati J, Miller JW, Gragoudas ES, Ng YS, D'Amore PA, Shima DT, Adamis AP: VEGF164-mediated inflammation is required for pathological, but not physiological, ischemia-induced retinal neovascularization. J Exp Med. 2003 Aug 4;198(3):483-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/12900522,*Mitnacht R, Bischof A, Torres-Nagel N, Hünig T: Opposite CD4/CD8 lineage decisions of CD4+8+ mouse and rat thymocytes to equivalent triggering signals: correlation with thymic expression of a truncated CD8 alpha chain in mice but not rats. J Immunol. 1998 Jan 15;160(2):700-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/9551905,*Hirji N, Lin TJ, Befus AD: A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production. J Immunol. 1997 Feb 15;158(4):1833-40., URL: https://www.ncbi.nlm.nih.gov/pubmed/9029123,*Torres-Nagel N, Kraus E, Brown MH, Tiefenthaler G, Mitnacht R, Williams AF, Hünig T: Differential thymus dependence of rat CD8 isoform expression. Eur J Immunol. 1992 Nov;22(11):2841-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/1358623,*Barclay AN: The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues. Immunology. 1981 Apr;42(4):593-600., URL: https://www.ncbi.nlm.nih.gov/pubmed/7016746

NCBI and Uniprot Product Information

• NCBI GI #: 13928726
• NCBI GeneID: 24930
• NCBI Accession #: NP_113726.1
• NCBI GenBank Nucleotide #: NM_031538.2
• UniProt Accession #: P07725
• Molecular Weight: 26,196 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD8 alpha chain
• NCBI Official Synonym Full Names: CD8a molecule
• NCBI Official Symbol: Cd8a
• NCBI Protein Information: T-cell surface glycoprotein CD8 alpha chain; CD8 antigen 32 kDa chain; CD8 antigen alpha-chain; CD8 antigen, alpha chain; CD8 antigen, alpha-chain; OX-8 membrane antigen
• UniProt Protein Name: T-cell surface glycoprotein CD8 alpha chain
• UniProt Gene Name: Cd8a
• UniProt Entry Name: CD8A_RAT