Mouse anti-Human CD95/FAS/TNFRSF6 Monoclonal Antibody | anti-FAS antibody

CD95/FAS/TNFRSF6

Cat. Num. AAA215036

• Gene Names: FAS; APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: CD95/FAS/TNFRSF6, Antibody; CD95/FAS/TNFRSF6; ALPS1A; Apoptosis APO1 antigen 1; Apoptosis-mediating surface antigen FAS; APT1; Delta Fas; Fas AMA; Fas cell surface death receptor; FAS1; FASL receptor; FASLG receptor; FASTM; Surface antigen APO1; Tumor necrosis factor receptor superfamily member 6 (TNFRSF6); anti-FAS antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone Number: FAS/3112
• Specificity: This MAb specifically recognizes CD95, also known as Fas, a transmembrane glycoprotein with a MW of 40-45kDa, containing 8kDa of N-glycoside-linked polysaccharide. It is a receptor for TNFSF6/FASLG, a member of the nerve growth factor receptor/tumor necrosis factor superfamily, mediating receptor-triggered apoptosis. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation, which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). CD95 antigen is expressed on the surface of various cell types, preferentially on the CD45RAlow CD45ROhigh subset of memory T lymphocytes.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 335

• Applicable Applications for anti-FAS antibody: IHC (Immunohistochemistry), ELISA
• Positive Control: MCF-7 cells (IF/FACS). Human hepatocellular or bladder carcinoma (IHC).
• Immunogen: Recombinant fragment (around aa26-96) of human CD95 (FAS) protein (exact sequence is proprietary)
• Cellular Localization: Cell Surface
• Hu-chromosome Location: 10q24.1
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Application Data

(Analysis of Protein Array containing more than 19,000 full-length human proteins using CD95 Mouse Monoclonal Antibody (FAS/3112). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

SDS-PAGE

(SDS-PAGE Analysis Purified CD95 Mouse Monoclonal Antibody (FAS/3112). Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human colon stained with CD95 Mouse Monoclonal Antibody (FAS/3112).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human colon stained with CD95 Mouse Monoclonal Antibody (FAS/3112).)

References

Sasaki et al. Br J Urol 81: 852, 1998.

NCBI and Uniprot Product Information

• NCBI GI #: 4507583
• NCBI GeneID: 355
• NCBI Accession #: NP_000034.1
• NCBI GenBank Nucleotide #: NP_000034.1
• UniProt Accession #: P25445
• Molecular Weight: 38-50kDa
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 6 isoform 1
• NCBI Official Synonym Full Names: Fas cell surface death receptor
• NCBI Official Symbol: FAS
• NCBI Official Synonym Symbols: APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 6
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 6
• UniProt Gene Name: FAS
• UniProt Synonym Gene Names: APT1; FAS1; TNFRSF6
• UniProt Entry Name: TNR6_HUMAN