IHC (Immunohistochemistry)
(CD99 Antibody in Immunohistochemistry (IHC (P)) -- Formalin-fixed, paraffin-embedded human Ewing's Sarcoma stained with CD99 Monoclonal Antibody (12E7).)
Mouse anti-Human CD99 / MIC2 Monoclonal Antibody | anti-CD99 antibody
CD99 / MIC2 (Ewings Sarcoma Marker) Mouse Monoclonal Antibody
Gene Names
CD99; MIC2; HBA71; MIC2X; MIC2Y; MSK5X
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Synonyms
CD99 / MIC2, Antibody; CD99 / MIC2 (Ewings Sarcoma Marker) Mouse Monoclonal Antibody; 12E7; E2 antigen; MIC 2X; MIC 2Y; MIC2; Protein MIC2; Surface antigen MIC2; T-cell surface glycoprotein E2; anti-CD99 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
12E7
Specificity
Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32kDa (p30/32). Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis. CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing's sarcoma cells and primitive peripheral neuroectodermal tumors.
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. (Also available WITHOUT BSA & azide at 1.0mg/ml.)
Sequence Length
169
Applicable Applications for anti-CD99 antibody
Flow Cytometry (FC), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
FC - 1-2ug/million cells
IF - 1-3ug/ml
IHC - 1-2ug/ml (NOTE: 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degree C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined by the end user.
IF - 1-3ug/ml
IHC - 1-2ug/ml (NOTE: 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degree C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined by the end user.
Cellular Localization
Membrane
Immunogen
Human acute lymphocytic leukemia T-cells
Positive Control
MOLT-4 cells. Pancreas or Ewings sarcoma
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Non-hazardous. No MSDS required.
Antibody without azide - store at -20 to -80 degree C.
Non-hazardous. No MSDS required.
IHC (Immunohistochemistry)
(CD99 Antibody in Immunohistochemistry (IHC (P)) -- Formalin-fixed, paraffin-embedded human Ewing's Sarcoma stained with CD99 Monoclonal Antibody (12E7).)
IHC (Immunohistochemistry)
(CD99 Antibody in Immunohistochemistry (IHC (P)) -- Formalin-fixed, paraffin-embedded human Ewing's Sarcoma stained with CD99 Monoclonal Antibody (12E7).)
SDS-PAGE
(CD99 Antibody in SDS-PAGE -- SDS-PAGE Analysis of Purified CD99 Mouse Monoclonal Antibody (12E7). Confirmation of Purity and Integrity of Antibody.)
SDS-PAGE
(CD99 Antibody in SDS-PAGE -- SDS-PAGE Analysis of Purified CD99 Mouse Monoclonal Antibody (12E7). Confirmation of Purity and Integrity of Antibody.)
References
Levy R, Dilley J, Fox RI, Warnke R. A human thymus-leukemia antigen defined by hybridoma monoclonal antibodies. PNAS USA 1979;76(12):6552-6
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27-32kDa
NCBI Official Full Name
CD99 antigen isoform b
NCBI Official Synonym Full Names
CD99 molecule
NCBI Official Symbol
CD99
NCBI Official Synonym Symbols
MIC2; HBA71; MIC2X; MIC2Y; MSK5X
NCBI Protein Information
CD99 antigen
UniProt Protein Name
CD99 antigen
UniProt Gene Name
CD99
UniProt Synonym Gene Names
MIC2; MIC2X; MIC2Y
UniProt Entry Name
CD99_HUMAN
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Product Notes
The CD99 cd99 (Catalog #AAA13840) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD99 / MIC2 (Ewings Sarcoma Marker) Mouse Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD99 / MIC2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC), Immunofluorescence (IF), Immunohistochemistry (IHC). FC - 1-2ug/million cells IF - 1-3ug/ml IHC - 1-2ug/ml (NOTE: 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degree C followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined by the end user. Researchers should empirically determine the suitability of the CD99 cd99 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD99 / MIC2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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