WB (Western Blot)
(Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with hydroxyurea (4mm, 24h, +)(line B); treated with hydroxyurea and lambda-phosphatase (line C) using Phospho-Cdc2 (Tyr15) Antibody, Rabbit MAb (#AAA259508) at 1:10000 dilution.)
cdc2 Recombinant Antibody | CDK1 recombinant antibody
Phospho-cdc2 (Tyr15) Antibody, Rabbit MAb
Reactivity
Human
Applications
Western Blot
Purity
Protein A
Synonyms
cdc2, Recombinant Antibody; Phospho-cdc2 (Tyr15) Antibody, Rabbit MAb; cyclin-dependent kinase 1; Anti-CDC2 Antibody; Anti-CDC28A Antibody; Anti-CDKN1 Antibody; Anti-P34CDC2 Antibody; CDK1 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
0019
Specificity
Human Phospho-cdc2 (Tyr15)Species predicted to react based on 100% sequence homology: Mouse, Rat, Cynomolgus, Bovin, Chicken, Fruit fly
Purity/Purification
Protein A
Form/Format
Liquid; 10 mM sodium HEPES, 150 mM NaCl, 100ug/mL BSA, 50% glycerol, pH 7.5
Applicable Applications for CDK1 recombinant antibody
WB (Western Blot)
Immunogen
A synthetic phosphopeptide corresponding to residues around Tyr15 of the Human cdc2
Preparation
This antibody was obtained from a rabbit immunized with a synthetic phosphopeptide corresponding to residues around Tyr15 of the Human cdc2.
Preparation and Storage
This antibody can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
WB (Western Blot)
(Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with hydroxyurea (4mm, 24h, +)(line B); treated with hydroxyurea and lambda-phosphatase (line C) using Phospho-Cdc2 (Tyr15) Antibody, Rabbit MAb (#AAA259508) at 1:10000 dilution.)
WB (Western Blot)
(Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with hydroxyurea (4mm, 24h, +)(line B); treated with hydroxyurea and lambda-phosphatase (line C) using Phospho-Cdc2 (Tyr15) Antibody, Rabbit MAb (#AAA259508) at 1:10000 dilution.)
WB (Western Blot)
(Western blot analysis of extracts from serum-starved Hela, untreated (-) or treated with hydroxyurea (4mm, 24h; +), using Phospho-Cdc2 (Tyr15) Antibody, Rabbit MAb (#AAA259508) at 1:10000 dilution(upper) or Anti-CDK1/CDC2 Antibody, Rabbit PAb at 1:2000 dilution (lower).)
WB (Western Blot)
(Western blot analysis of extracts from serum-starved Hela, untreated (-) or treated with hydroxyurea (4mm, 24h; +), using Phospho-Cdc2 (Tyr15) Antibody, Rabbit MAb (#AAA259508) at 1:10000 dilution(upper) or Anti-CDK1/CDC2 Antibody, Rabbit PAb at 1:2000 dilution (lower).)
Related Product Information for CDK1 recombinant antibody
CDC2, also known as CDK1, contains 1 protein kinase domain and belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily. CDC2 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with CDC2 and function as regulatory subunits. The kinase activity of CDK1 is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of CDC2 also play important regulatory roles in cell cycle control. It is required in higher cells for entry into S-phase and mitosis. CDC2 also is a cyclin-dependent kinase which displays CTD kinase activity and is required for RNA splicing. It has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. CDK1 is required for RNA splicing, possibly by phosphorylating SRSF1/SF2. It is involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogn inhibitors.
References
Le. MG. e. al. (1987. Complementatio. use. t. clon. . huma. homologu. o. th. fissio. yeas. cel. cycl. contro. gen. cdc2. Nature. 327(6117):31-5.Enserin. JM. e. al. (2010. A. overvie. o. Cdk1-controlle. target. an. processes. Cel. Division. 5(11). 1-41.Ninomiya-Tsuj. J. e. al. (1991. Clonin. o. . huma. cDN. encodin. . CDC2-relate. kinas. b. complementatio. o. . buddin. yeas. cdc2. mutation. Pro. Nat. Aca. Sci. 88(20):9006-10.Zha. Q. e. al. (1999. Associatio. wit. Cdc. an. inhibitio. o. Cdc2/Cycli. B. kinas. activit. b. th. p53-regulate. protei. Gadd45. Oncogene. 18(18):2892-900.Ji. S. e. al. (2000. Th. GADD4. inhibitio. o. Cdc. kinas. correlate. wit. GADD45-mediate. growt. suppression. . Bio. Chem. 275(22):16602-8.
Similar Products
Product Notes
The CDK1 (Catalog #AAA259508) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-cdc2 (Tyr15) Antibody, Rabbit MAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's cdc2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the CDK1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "cdc2, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.