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FCM (Flow Cytometry)
(Flow cytometric analysis of K562 cells with CDC40 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Rabbit CDC40 Monoclonal Antibody | anti-CDC40 antibody
CDC40 Antibody
Gene Names
CDC40; EHB3; PRP17; PRPF17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
CDC40; Monoclonal Antibody; CDC40 Antibody; Cdc40 antibody; Cell division cycle 40 antibody; Cell division cycle 40 homolog antibody; EH binding protein 3 antibody; EH-binding protein 3 antibody; Ehb3 antibody; hPRP17 antibody; Pre mRNA processing factor 17 antibody; Pre mRNA splicing factor 17 antibody; Pre-mRNA-processing factor 17 antibody; PRP17 antibody; PRP17 homolog antibody; PRP17_HUMAN antibody; PRPF17 antibody; anti-CDC40 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
SD085-9
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-CDC40 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-5000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of K562 cells with CDC40 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
FCM (Flow Cytometry)
(Flow cytometric analysis of K562 cells with CDC40 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining CDC40 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining CDC40 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining CDC40 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining CDC40 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining CDC40 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining CDC40 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CDC40 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CDC40 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-CDC40 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-CDC40 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CDC40 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CDC40 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of CDC40 on Hela cells lysates using anti-CDC40 antibody at 1/2, 000 dilution.)
WB (Western Blot)
(Western blot analysis of CDC40 on Hela cells lysates using anti-CDC40 antibody at 1/2, 000 dilution.)
Related Product Information for anti-CDC40 antibody
Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division control (Cdc) genes are required at various points in the cell cycle. Cdc40, also known as pre-mRNA-processing factor 17 (PRPF17) or EH-binding protein 3 (EHB3), is a 579 amino acid nuclear protein. Cdc40 is essential for the catalytic step II of the pre-mRNA splicing process, in which Cdc40 associates with the spliceosome C complex. Cdc40 contains seven WD repeats, which are important in protein-protein interactions. Cdc40 has sequence similarity to the yeast protein Prp17, which is involved in pre-mRNA splicing and cell cycle progression. The sequence similarity between the mammalian Cdc40 and the yeast Prp17 may indicate an additional role in cell cycle progression for mammalian Cdc40.
Product Categories/Family for anti-CDC40 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
65,521 Da
NCBI Official Full Name
pre-mRNA-processing factor 17
NCBI Official Synonym Full Names
cell division cycle 40
NCBI Official Symbol
CDC40
NCBI Official Synonym Symbols
EHB3; PRP17; PRPF17
NCBI Protein Information
pre-mRNA-processing factor 17; hPRP17; PRP17 homolog; EH-binding protein 3; pre-mRNA splicing factor 17; cell division cycle 40 homolog
UniProt Protein Name
Pre-mRNA-processing factor 17
UniProt Gene Name
CDC40
UniProt Synonym Gene Names
EHB3; PRP17; PRPF17; Ehb3; hPRP17
UniProt Entry Name
PRP17_HUMAN
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Product Notes
The CDC40 cdc40 (Catalog #AAA30215) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDC40 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CDC40 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:1000-5000 IHC: 1:50-1:200 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the CDC40 cdc40 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDC40, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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