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Application Data
(Knockout Validation: Lane 1: Wild-type Jurkat cell lysate; Lane 2: FAK knockout Jurkat cell lysate; Predicted MW: 39,48,63,100,120kd Observed MW: 120kd Primary Ab: 1ug/ml Rabbit Anti-Human FAK Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Rabbit anti-Human Focal Adhesion Kinase (FAK) Polyclonal Antibody | anti-FAK antibody
Polyclonal Antibody to Focal Adhesion Kinase (FAK)
Gene Names
PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry
Purity
Affinity Chromatography
Synonyms
Focal Adhesion Kinase (FAK), Antibody; Polyclonal Antibody to Focal Adhesion Kinase (FAK); anti-FAK antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against FAK. It has been selected for its ability to recognize FAK in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
0.75mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- GIG QVLPTHLMEE RLIRQQQEME EDQRWLEKEE RFLKPDVRLS RGSIDREDGS LQGPIGNQHI YQPVGKPDPA APPKKPPRPG APGHLGSLAS LSSPADSYNE GVKLQPQEIS PPPTANLDRS NDKVYENVTG LVKAVIEMSS KIQPAPPEEY VPMVKEVGLA LRTLLATVDE TIPLLPASTH REIEMAQKLL NSDLGELINK
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- GIG QVLPTHLMEE RLIRQQQEME EDQRWLEKEE RFLKPDVRLS RGSIDREDGS LQGPIGNQHI YQPVGKPDPA APPKKPPRPG APGHLGSLAS LSSPADSYNE GVKLQPQEIS PPPTANLDRS NDKVYENVTG LVKAVIEMSS KIQPAPPEEY VPMVKEVGLA LRTLLATVDE TIPLLPASTH REIEMAQKLL NSDLGELINK
Sequence Length
1052
Applicable Applications for anti-FAK antibody
WB (Western Blot), ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry)
Immunogen
Recombinant FAK (Gly798~Ala1041) expressed in E.coli.
Cross Reactivity
Human
Conjugated Antibody
The APC conjugated antibody version of this item is also available as
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Application Data
(Knockout Validation: Lane 1: Wild-type Jurkat cell lysate; Lane 2: FAK knockout Jurkat cell lysate; Predicted MW: 39,48,63,100,120kd Observed MW: 120kd Primary Ab: 1ug/ml Rabbit Anti-Human FAK Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Application Data
(Knockout Validation: Lane 1: Wild-type Jurkat cell lysate; Lane 2: FAK knockout Jurkat cell lysate; Predicted MW: 39,48,63,100,120kd Observed MW: 120kd Primary Ab: 1ug/ml Rabbit Anti-Human FAK Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Thyroid Tissue))
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Thyroid Tissue))
IHC (Immunohistochemisry)
(DAB staining on fromalin fixed paraffin- embedded liver tissue))
IHC (Immunohistochemisry)
(DAB staining on fromalin fixed paraffin- embedded liver tissue))
WB (Western Blot)
(Western Blot: Sample: Recombinant protein.)
WB (Western Blot)
(Western Blot: Sample: Recombinant protein.)
WB (Western Blot)
(Western Blot: Sample: Human Lung lysate; Primary Ab: 1ug/ml Rabbit Anti-Human FAK Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot: Sample: Human Lung lysate; Primary Ab: 1ug/ml Rabbit Anti-Human FAK Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Product Categories/Family for anti-FAK antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
114,253 Da
NCBI Official Full Name
focal adhesion kinase 1 isoform c
NCBI Official Synonym Full Names
protein tyrosine kinase 2
NCBI Official Symbol
PTK2
NCBI Official Synonym Symbols
FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK
NCBI Protein Information
focal adhesion kinase 1
UniProt Protein Name
Focal adhesion kinase 1
UniProt Gene Name
PTK2
UniProt Synonym Gene Names
FAK; FAK1; FADK 1; FRNK; PPP1R71
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Product Notes
The FAK ptk2 (Catalog #AAA134986) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Focal Adhesion Kinase (FAK) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Focal Adhesion Kinase (FAK) can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the FAK ptk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- GIG QVLPTHLMEE RLIRQQQEME EDQRWLEKEE RFLKPDVRLS RGSIDREDGS LQGPIGNQHI YQPVGKPDPA APPKKPPRPG APGHLGSLAS LSSPADSYNE GVKLQPQEIS PPPTANLDRS NDKVYENVTG LVKAVIEMSS KIQPAPPEEY VPMVKEVGLA LRTLLATVDE TIPLLPASTH REIEMAQKLL NSDLGELINK. It is sometimes possible for the material contained within the vial of "Focal Adhesion Kinase (FAK), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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