Application Data
(Western blot detection of CDK5(N-terminus) antibody in Jurkat,K562&Hela lysates using CDK5(N-terminus) antibody (1:1000 diluted). Predicted band size: 36KDa Observed band size: 36KDa.)
Mouse anti-Human CDK5 Monoclonal Antibody | anti-CDK5 antibody
CDK5 Antibody
Gene Names
CDK5; LIS7; PSSALRE
Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Purity
Affinity purified
Synonyms
CDK5, Antibody; CDK5 Antibody; PSSALRE; anti-CDK5 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Specificity
This antibody detects endogenous levels CDK5(N-terminus) and does not cross-react with related proteins.
Purity/Purification
Affinity purified
Form/Format
Purified in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%, glycerol
Sequence Length
260
Applicable Applications for anti-CDK5 antibody
ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
Purified recombinant human CDK5(N-terminus) protein fragments expressed in E Coli.
Cellular Localization
Cytoplasm; Cell Membrane; Peripheral membrane protein; Perikaryon; Cell projection
Application Data
(Western blot detection of CDK5(N-terminus) antibody in Jurkat,K562&Hela lysates using CDK5(N-terminus) antibody (1:1000 diluted). Predicted band size: 36KDa Observed band size: 36KDa.)
Application Data
(Western blot detection of CDK5(N-terminus) antibody in Jurkat,K562&Hela lysates using CDK5(N-terminus) antibody (1:1000 diluted). Predicted band size: 36KDa Observed band size: 36KDa.)
Application Data
(Immunocytochemistry of HeLa cells using anti- CDK5(N-terminus) antibody diluted 1:150.)
Application Data
(Immunocytochemistry of HeLa cells using anti- CDK5(N-terminus) antibody diluted 1:150.)
Related Product Information for anti-CDK5 antibody
UniPort Summary Function: Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase.
Entrez Summary: Cdks (cyclin-dependent kinases) are heteromeric serine/threonine kinases that control progression through the cell cycle in concert with their regulatory subunits, the cyclins. Although there are 12 different cdk genes, only 5 have been shown to directly drive the cell cycle (Cdk1,-2,-3,-4, and-6). Following extracellular mitogenic stimuli, cyclin D gene expression is upregulated. Cdk4 forms a complex with cyclin D and phosphorylates Rb protein, leading to liberation of the transcription factor E2F. E2F induces transcription of genes including cyclins A and E, DNA polymerase and thymidine kinase. Cdk4-cyclin E complexes form and initiate G1/S transition. Subsequently, Cdk1-cyclin B complexes form and induce G2/M phase transition. Cdk1-cyclin B activation induces the breakdown of the nuclear envelope and the initiation of mitosis. Cdks are constitutively expressed and are regulated by several kinases and phosphastases, including Wee1, CDK-activating kinase and Cdc25 phosphatase. In addition, cyclin expression is induced by molecular signals at specific points of the cell cycle, leading to activation of Cdks. Tight control of Cdks is essential as misregulation can induce unscheduled proliferation, and genomic and chromosomal instability. Cdk4 has been shown to be mutated in some types of cancer, whilst a chromosomal rearrangement can lead to Cdk6 overexpression in lymphoma, leukemia and melanoma. Cdks are currently under investigation as potential targets for antineoplastic therapy, but as Cdks are essential for driving each cell cycle phase, therapeutic strategies that block Cdk activity are unlikely to selectively target tumor cells.
Entrez Summary: Cdks (cyclin-dependent kinases) are heteromeric serine/threonine kinases that control progression through the cell cycle in concert with their regulatory subunits, the cyclins. Although there are 12 different cdk genes, only 5 have been shown to directly drive the cell cycle (Cdk1,-2,-3,-4, and-6). Following extracellular mitogenic stimuli, cyclin D gene expression is upregulated. Cdk4 forms a complex with cyclin D and phosphorylates Rb protein, leading to liberation of the transcription factor E2F. E2F induces transcription of genes including cyclins A and E, DNA polymerase and thymidine kinase. Cdk4-cyclin E complexes form and initiate G1/S transition. Subsequently, Cdk1-cyclin B complexes form and induce G2/M phase transition. Cdk1-cyclin B activation induces the breakdown of the nuclear envelope and the initiation of mitosis. Cdks are constitutively expressed and are regulated by several kinases and phosphastases, including Wee1, CDK-activating kinase and Cdc25 phosphatase. In addition, cyclin expression is induced by molecular signals at specific points of the cell cycle, leading to activation of Cdks. Tight control of Cdks is essential as misregulation can induce unscheduled proliferation, and genomic and chromosomal instability. Cdk4 has been shown to be mutated in some types of cancer, whilst a chromosomal rearrangement can lead to Cdk6 overexpression in lymphoma, leukemia and melanoma. Cdks are currently under investigation as potential targets for antineoplastic therapy, but as Cdks are essential for driving each cell cycle phase, therapeutic strategies that block Cdk activity are unlikely to selectively target tumor cells.
Product Categories/Family for anti-CDK5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36kDa
NCBI Official Full Name
cyclin-dependent-like kinase 5 isoform 2
NCBI Official Synonym Full Names
cyclin dependent kinase 5
NCBI Official Symbol
CDK5
NCBI Official Synonym Symbols
LIS7; PSSALRE
NCBI Protein Information
cyclin-dependent-like kinase 5
UniProt Protein Name
Cyclin-dependent-like kinase 5
UniProt Gene Name
CDK5
UniProt Synonym Gene Names
CDKN5; TPKII catalytic subunit
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Product Notes
The CDK5 cdk5 (Catalog #AAA111277) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CDK5 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CDK5 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CDK5 cdk5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDK5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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