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product-image-AAA215197_SDS_PAGE11.jpg SDS-PAGE (SDS-PAGE Analysis Purified CDw75 Mouse Monoclonal Antibody (ZB55).Confirmation of Purity and Integrity of Antibody.)

Mouse anti-Human CDw75 Monoclonal Antibody | anti-CDw75 antibody

CDw75 (B-Cell Marker)

Gene Names
ST6GAL1; ST6N; SIAT1; ST6GalI
Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA at 1.0mg/ml
Synonyms
CDw75, Antibody; CDw75 (B-Cell Marker); 6-sialyltransferase 1 (6-ST1); Alpha 2;6 ST1; Beta galactoside alpha 2;6 sialyltransferase 1; CMP N acetylneuraminate beta galactosamide alpha 2;6 sialyltransferase 1; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2; SIAT1; ST6 beta galactosamide alpha 2;6 sialyltranferase 1; ST6Gal I; ST6N; anti-CDw75 antibody
Ordering
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgM, kappa
Clone Number
ZB55
Specificity
Recognizes a neuraminidase-sensitive sialoprotein (CDw75), present on cell membrane and cytoplasm of germinal center B-cells and derived lymphomas. This MAb reacts with RBC precursors of bone marrow, ductal and ciliated epithelial cells of kidney, breast, prostate, pancreas, lung, and with glioblastomas, astrocytomas, and Reed Sternberg cells in lymphocyte predominant Hodgkin's disease. It is shown to be a helpful antibody for ascribing a B-cell phenotype in known lymphoid tissues.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA at 1.0mg/ml
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by PEG precipitation. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
406
Applicable Applications for anti-CDw75 antibody
IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Positive Control
HeLa or Daudi cells (IF/FACS). Germinal center B- cells in a lymph node or tonsil (IHC). Use LN1 s ability to stain surface of erythrocytes as an internal positive control.
Immunogen
CD32 peptitdes
Cellular Localization
Cell Surface and Cytoplasmic
Hu-chromosome Location
3q27.3
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis Purified CDw75 Mouse Monoclonal Antibody (ZB55).Confirmation of Purity and Integrity of Antibody.)

product-image-AAA215197_SDS_PAGE11.jpg SDS-PAGE (SDS-PAGE Analysis Purified CDw75 Mouse Monoclonal Antibody (ZB55).Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded normal human spleen tissue stained with CDw75 Mouse Monoclonal Antibody (ZB55).)

product-image-AAA215197_IHC13.jpg IHC (Immunohiostchemistry) (Formalin-fixed, paraffin-embedded normal human spleen tissue stained with CDw75 Mouse Monoclonal Antibody (ZB55).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded normal human spleen tissue stained with CDw75 Mouse Monoclonal Antibody (ZB55).)

product-image-AAA215197_IHC15.jpg IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded normal human spleen tissue stained with CDw75 Mouse Monoclonal Antibody (ZB55).)
References
1. Marder RJ et. al. Lab Invest 52: 497-504, 1985. 2. Epstein AL et. al. J of Immunology 133: 1028-1036, 1984.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
46kDa
NCBI Official Full Name
beta-galactoside alpha-2,6-sialyltransferase 1 isoform a
NCBI Official Synonym Full Names
ST6 beta-galactoside alpha-2,6-sialyltransferase 1
NCBI Official Symbol
ST6GAL1
NCBI Official Synonym Symbols
ST6N; SIAT1; ST6GalI
NCBI Protein Information
beta-galactoside alpha-2,6-sialyltransferase 1
UniProt Protein Name
Beta-galactoside alpha-2,6-sialyltransferase 1
UniProt Gene Name
ST6GAL1
UniProt Synonym Gene Names
SIAT1; Alpha 2,6-ST 1; ST6GalI
UniProt Entry Name
SIAT1_HUMAN

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Product Notes

The CDw75 st6gal1 (Catalog #AAA215197) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CDw75 (B-Cell Marker) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CDw75 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CDw75 st6gal1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDw75, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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