Mouse anti-Human CDX2/Caudal Type Homeobox 2 Monoclonal Antibody | anti-CDX2 antibody

CDX2/Caudal Type Homeobox 2 (GI Epithelial Marker)

Cat. Num. AAA214879

• Gene Names: CDX2; CDX3; CDX-3; CDX2/AS
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: CDX2/Caudal Type Homeobox 2, Antibody; CDX2/Caudal Type Homeobox 2 (GI Epithelial Marker); Caudal type homeobox 2; Caudal type homeobox transcription factor 2; Caudal-type homeobox protein 2; CDX2; anti-CDX2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: CDX2/2214
• Specificity: The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. The intestine-specific transcription factors CDX1 and CDX2 are important for directing intestinal development, differentiation, proliferation and maintenance of the intestinal phenotype. CDX2 protein expression has been seen in GI carcinomas. Anti-CDX2 has been useful to establish GI origin of metastatic adenocarcinomas and carcinoidsand is especially useful to distinguish metastatic colorectal adenocarcinoma from lung adenocarcinoma.However, mucinous carcinomas of the ovary also express CDX2 protein. It limits the usefulness of this marker in the distinction of metastatic colorectal adenocarcinoma from mucinous carcinoma of the ovary.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 313

• Applicable Applications for anti-CDX2 antibody: IHC (Immunohistochemistry)
• Positive Control: HT29 cells. Colon Carcinoma.
• Immunogen: Recombinant human full-length CDX2 protein
• Cellular Localization: Nuclear
• Hu-chromosome Location: 13q12.2
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Application Data

(Analysis of Protein Array containing more than 19,000 full-length human proteins using CDX2 Mouse Monoclonal Antibody (CDX2/2214). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.)

SDS-PAGE

(SDS-PAGE Analysis Purified CDX2 Mouse Monoclonal Antibody (CDX2/2214). Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CDX2 Mouse Monoclonal Antibody (CDX2/2214).)

References

1. Silberg, D.G., et al. 2000. Gastroenterology 119: 961-971. 2. Mallo, G.V., et al. 1998. J. Biol. Chem. 273: 14030-14036.

NCBI and Uniprot Product Information

• NCBI GI #: 372624392
• NCBI GeneID: 1045
• NCBI Accession #: NP_001256.3
• NCBI GenBank Nucleotide #: NP_001256.3
• UniProt Accession #: Q99626
• Molecular Weight: 40kDa
• NCBI Official Full Name: homeobox protein CDX-2 isoform 1
• NCBI Official Synonym Full Names: caudal type homeobox 2
• NCBI Official Symbol: CDX2
• NCBI Official Synonym Symbols: CDX3; CDX-3; CDX2/AS
• NCBI Protein Information: homeobox protein CDX-2
• UniProt Protein Name: Homeobox protein CDX-2
• UniProt Gene Name: CDX2
• UniProt Synonym Gene Names: CDX3
• UniProt Entry Name: CDX2_HUMAN