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WB (Western Blot)
(Detection of human CEACAM1/5 by western blot. Samples: Whole cell lysate (5 ug) from HEK293T, UACC-257, GaMG, Hep-G2, U2OS, A-549, SK-MEL-28, MCF-7, and HT-29 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CEACAM1/5 recombinant monoclonal antibody (AAA213541 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds.)
CEACAM1/5 recombinant antibody
Rabbit anti-CEACAM1/5 Recombinant Monoclonal Antibody [BLR032F]
Gene Names
CEACAM1; BGP; BGP1; BGPI
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
CEACAM1/5, Recombinant Antibody; Rabbit anti-CEACAM1/5 Recombinant Monoclonal Antibody [BLR032F]; BGP1; CD66a antigen; CD antigen CD66a; carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein); carcinoembryonic antigen-related cell adhesion molecule 1; Biliary glycoprotein 1; BGP-1; BGP; antigen CD66; BGPI; Carcinoembryonic antigen; carcinoembryonic antigen-related cell adhesion molecule 5; CD antigen CD66e; CD66e; CEA; meconium antigen 100; Carcinoembryonic antigen-related cell adhesion molecule 1; carcinoembryonic antigen related cell adhesion molecule 1; CEACAM1/5 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR032F
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
50ug/ml (varies by lot)
Applicable Applications for CEACAM1/5 recombinant antibody
FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Residues 35-433 (ECD)
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CEACAM1/5 by western blot. Samples: Whole cell lysate (5 ug) from HEK293T, UACC-257, GaMG, Hep-G2, U2OS, A-549, SK-MEL-28, MCF-7, and HT-29 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CEACAM1/5 recombinant monoclonal antibody (AAA213541 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds.)
WB (Western Blot)
(Detection of human CEACAM1/5 by western blot. Samples: Whole cell lysate (5 ug) from HEK293T, UACC-257, GaMG, Hep-G2, U2OS, A-549, SK-MEL-28, MCF-7, and HT-29 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CEACAM1/5 recombinant monoclonal antibody (AAA213541 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human CEACAM1/5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 2% of IP loaded) from UACC-257 cells. Antibodies: Rabbit anti-CEACAM1/5 recombinant monoclonal antibody (AAA213541 lot 2) used for IP at 20 ul/mg lysate. CEACAM1/5 was also immunoprecipitated by a previous lot of this antibody (AAA213541-1). For blotting immunoprecipitated CEACAM1/5, AAA213541 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human CEACAM1/5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 2% of IP loaded) from UACC-257 cells. Antibodies: Rabbit anti-CEACAM1/5 recombinant monoclonal antibody (AAA213541 lot 2) used for IP at 20 ul/mg lysate. CEACAM1/5 was also immunoprecipitated by a previous lot of this antibody (AAA213541-1). For blotting immunoprecipitated CEACAM1/5, AAA213541 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemisry)
(Detection of human CEACAM 1/5 in FFPE colon carcinoma by immunohistochemistry. Antibody: Rabbit anti-CEACAM 1/5 recombinant monoclonal antibody (AAA213541 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemisry)
(Detection of human CEACAM 1/5 in FFPE colon carcinoma by immunohistochemistry. Antibody: Rabbit anti-CEACAM 1/5 recombinant monoclonal antibody (AAA213541 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human CEACAM 1/5 in FFPE HT-29 by immunocytochemistry. Antibody: Rabbit anti-CEACAM 1/5 recombinant monoclonal antibody (AAA213541 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human CEACAM 1/5 in FFPE HT-29 by immunocytochemistry. Antibody: Rabbit anti-CEACAM 1/5 recombinant monoclonal antibody (AAA213541 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM/FACS (Flow Cytometry)
(Detection of human CEACAM1/5 (shaded) in HT-29 cells by flow cytometry. Antibody: Rabbit anti-CEACAM1/5 recombinant monoclonal antibody (AAA213541 lot 2) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of human CEACAM1/5 (shaded) in HT-29 cells by flow cytometry. Antibody: Rabbit anti-CEACAM1/5 recombinant monoclonal antibody (AAA213541 lot 2) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Related Product Information for CEACAM1/5 recombinant antibody
Carcinoembryonic antigen related cell adhesion molecules 1 and 5 (CEACAM1 and CEACAM5) are members of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. CEACAM1 was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. CEACAM5 is used as a clinical biomarker for gastrointestinal cancers and may promote tumor development through its role as a cell adhesion molecule. Additionally, CEACAM5 may regulate differentiation, apoptosis, and cell polarity.
Product Categories/Family for CEACAM1/5 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,871 Da
NCBI Official Full Name
carcinoembryonic antigen-related cell adhesion molecule 1 isoform 2
NCBI Official Synonym Full Names
carcinoembryonic antigen related cell adhesion molecule 1
NCBI Official Symbol
CEACAM1
NCBI Official Synonym Symbols
BGP; BGP1; BGPI
NCBI Protein Information
carcinoembryonic antigen-related cell adhesion molecule 1
UniProt Protein Name
Carcinoembryonic antigen-related cell adhesion molecule 1
UniProt Gene Name
CEACAM1
UniProt Synonym Gene Names
BGP; BGP1; BGP-1
UniProt Entry Name
CEAM1_HUMAN
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Product Notes
The CEACAM1/5 ceacam1 (Catalog #AAA213541) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CEACAM1/5 Recombinant Monoclonal Antibody [BLR032F] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CEACAM1/5 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CEACAM1/5 ceacam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CEACAM1/5, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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