Application Data
(Fig3: Members of the CEA family were expressed on BOSC cells after transient transfection with expression vectors containing either the cDNA of CEACAM1,5,6,7,8,19,20 or 21. Recognition of CEACAM3 and 4 was tested on stably transfected HeLa (CEACAM3) and CHO cells (CEACAM4). Expression of the constructs was confirmed with monoclonal antibodies known to recognise the corresponding proteins (CEACAM1,3,4: D14HD11; CEACAM5: 26/3/13; CEACAM6: 9A6; CEACAM7: BAC2; CEACAM8: GM-2H6; CEACAM19-21: anti-myc; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G-purified GM-8G5 was tested on all CEACAM transfectants. A positive signal was only obtained with CEACAM1-expressing cells (red curves).)
Mouse anti-Human CEACAM1 Monoclonal Antibody | anti-CEACAM1 antibody
anti-human CEACAM1 monoclonal antibody
Gene Names
CEACAM1; BGP; BGP1; BGPI
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Flow Cytometry, Functional Assay
Purity
Protein G
Synonyms
CEACAM1, Antibody; anti-human CEACAM1 monoclonal antibody; CD66a; BGP; biliary glycoprotein I; anti-CEACAM1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1/kappa
Clone Number
GM-8G5
Specificity
anti-human CEACAM1 (biliary glycoprotein I, BGP/CD66a)
Purity/Purification
Protein G
Form/Format
Purified; Phosphate buffered saline, pH 7.2
Sequence Length
464
Applicable Applications for anti-CEACAM1 antibody
IHC (Immunohistochemistry), ELISA (Competitive ELISA), FCM/FACS (Flow Cytometry)
Immunogen
Genetic immunization with cDNA encoding the extracellular region of human CEACAM1-A2
Selection
based on recognition of the complete native protein expressed on transfected mammalian cells
Preparation and Storage
Short term: 2 degree C - 8 degree C
Long term: -20 degree C
Avoid repeated freezing and thawing
Long term: -20 degree C
Avoid repeated freezing and thawing
Application Data
(Fig3: Members of the CEA family were expressed on BOSC cells after transient transfection with expression vectors containing either the cDNA of CEACAM1,5,6,7,8,19,20 or 21. Recognition of CEACAM3 and 4 was tested on stably transfected HeLa (CEACAM3) and CHO cells (CEACAM4). Expression of the constructs was confirmed with monoclonal antibodies known to recognise the corresponding proteins (CEACAM1,3,4: D14HD11; CEACAM5: 26/3/13; CEACAM6: 9A6; CEACAM7: BAC2; CEACAM8: GM-2H6; CEACAM19-21: anti-myc; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G-purified GM-8G5 was tested on all CEACAM transfectants. A positive signal was only obtained with CEACAM1-expressing cells (red curves).)
Application Data
(Fig3: Members of the CEA family were expressed on BOSC cells after transient transfection with expression vectors containing either the cDNA of CEACAM1,5,6,7,8,19,20 or 21. Recognition of CEACAM3 and 4 was tested on stably transfected HeLa (CEACAM3) and CHO cells (CEACAM4). Expression of the constructs was confirmed with monoclonal antibodies known to recognise the corresponding proteins (CEACAM1,3,4: D14HD11; CEACAM5: 26/3/13; CEACAM6: 9A6; CEACAM7: BAC2; CEACAM8: GM-2H6; CEACAM19-21: anti-myc; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G-purified GM-8G5 was tested on all CEACAM transfectants. A positive signal was only obtained with CEACAM1-expressing cells (red curves).)
Application Data
(Fig.2: Immunohistochemical staining on cryosections of colon tissue using AAA71485. PBS containing 2.5% horse serum served as negative control (A). Detection of GM-8G5 occurred with a biotinylated anti-mouse-IgG secondary antibody and a streptavidin-peroxidase conjugate (B). Diaminobenzidine was used as substrate. Nuclei were stained with hematoxilin.)
Application Data
(Fig.2: Immunohistochemical staining on cryosections of colon tissue using AAA71485. PBS containing 2.5% horse serum served as negative control (A). Detection of GM-8G5 occurred with a biotinylated anti-mouse-IgG secondary antibody and a streptavidin-peroxidase conjugate (B). Diaminobenzidine was used as substrate. Nuclei were stained with hematoxilin.)
Application Data
(Fig.1: FACS analysis of BOSC23 cells using AAA71485. BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM1 (red curve) or an irrelevant protein (control transfectant: black curve). Binding of GM-8G5 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with CEACAM1 transfected cells.)
Application Data
(Fig.1: FACS analysis of BOSC23 cells using AAA71485. BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM1 (red curve) or an irrelevant protein (control transfectant: black curve). Binding of GM-8G5 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with CEACAM1 transfected cells.)
Related Product Information for anti-CEACAM1 antibody
Background: CEACAM1 (BGP/CD66a) is a transmembrane glycoprotein which belongs to the carcinoembryonic antigen (CEA) gene family (1,2). It is expressed on cells of epithelial and myeloid origin and mediates homophilic intercellular interactions that influence cellular growth, immune cell activation, and tissue morphogenesis. CEACAM1 is a putative tumour suppressor based on diminished expression in some aggressive types of cancer cells (3). The anti-tumour effect may be due to inhibition of tumour angiogenesis, possibly by increased secretion of anti-angiogenic molecules from the cells (4). Like all members of the CEACAM family, it consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by two immunoglobulin constant-like A (A1, A2) and one B domain. While the N, A1 and B domains can also be found in other CEA-family members, the A2 domain of CEACAM1 differs from those found in other CEACAM.
References
Zimmermann W (2002). Carcinoembryonic antigen. In Wiley Encyclopedia of Molecular Medicine (T. Creighton, ed.), John Wiley & Sons Inc., New York, USA, pp. 459-462.
Hammarström S (1999). The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues. Semin. Cancer Biol. 9, 67-81.
Luo W, Tapolsky M, Early K, Wood CG, Wilson DR, Logothetis CJ, Lin SH (1999). Tumorsuppressive activity of CD66a in prostate cancer. Cancer Gene Ther. 6(4): 313-21.
Tilki D, Irmak S, Oliveira-Ferrer L, Hauschild J, Miethe K, Atakaya H, Hammerer P, Friedrich MG, Schuch G, Galalae R, Stief CG, Kilic E, Huland H, Ergun S (2006). CEA-related cell adhesion molecule-1 is involved in angiogenic switch in prostate cancer. Oncogene 25(36):4965- 74
Hammarström S (1999). The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues. Semin. Cancer Biol. 9, 67-81.
Luo W, Tapolsky M, Early K, Wood CG, Wilson DR, Logothetis CJ, Lin SH (1999). Tumorsuppressive activity of CD66a in prostate cancer. Cancer Gene Ther. 6(4): 313-21.
Tilki D, Irmak S, Oliveira-Ferrer L, Hauschild J, Miethe K, Atakaya H, Hammerer P, Friedrich MG, Schuch G, Galalae R, Stief CG, Kilic E, Huland H, Ergun S (2006). CEA-related cell adhesion molecule-1 is involved in angiogenic switch in prostate cancer. Oncogene 25(36):4965- 74
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
carcinoembryonic antigen-related cell adhesion molecule 1 isoform 2
NCBI Official Synonym Full Names
carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)
NCBI Official Symbol
CEACAM1
NCBI Official Synonym Symbols
BGP; BGP1; BGPI
NCBI Protein Information
carcinoembryonic antigen-related cell adhesion molecule 1; antigen CD66; CD66a antigen
UniProt Gene Name
CEACAM1
UniProt Synonym Gene Names
BGP; BGP1; BGP-1
UniProt Entry Name
CEAM1_HUMAN
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Product Notes
The CEACAM1 ceacam1 (Catalog #AAA71485) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CEACAM1 monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CEACAM1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA (Competitive ELISA), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CEACAM1 ceacam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CEACAM1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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