Application Data
(SDS-PAGE analysis of HT-12D8 The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Fig.4). Fig.4: SDS-PAGE analysis of purified HT-12D8 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified HT-12D8 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.)
Mouse anti-Human CEACAM20 Monoclonal Antibody | anti-CEACAM20 antibody
anti-human CEACAM20 monoclonal antibody
Application Data
(SDS-PAGE analysis of HT-12D8 The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Fig.4). Fig.4: SDS-PAGE analysis of purified HT-12D8 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified HT-12D8 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.)
Application Data
(SDS-PAGE analysis of HT-12D8 The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Fig.4). Fig.4: SDS-PAGE analysis of purified HT-12D8 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified HT-12D8 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.)
Application Data
(Fig3: BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3-8 or CEACAM19-21. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1,3,4,5 and 6: D14HD11; CEACAM7: BAC2; CEACAM8: Tet2; CEACAM19,21: ?-myc; CEACAM20: ?-flag; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G-purified HT-12D8 was tested on all CEACAM transfectants. A positive signal was obtained only with CEACAM20 transfected cells (red curve).)
Application Data
(Fig3: BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3-8 or CEACAM19-21. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1,3,4,5 and 6: D14HD11; CEACAM7: BAC2; CEACAM8: Tet2; CEACAM19,21: ?-myc; CEACAM20: ?-flag; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G-purified HT-12D8 was tested on all CEACAM transfectants. A positive signal was obtained only with CEACAM20 transfected cells (red curve).)
Application Data
(Fig.2: Spectral Confocal Microscopy of CHO cells using HT-12D8 Cat.# GM-0516. CHO cells were transiently transfected with an expression vector encoding CEACAM20. Binding of HT-12D8 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).)
Application Data
(Fig.2: Spectral Confocal Microscopy of CHO cells using HT-12D8 Cat.# GM-0516. CHO cells were transiently transfected with an expression vector encoding CEACAM20. Binding of HT-12D8 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).)
Application Data
(Fig.1: FACS analysis of BOSC23 cells using HT-12D8 Cat.# GM-0516. BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM20 (red curve) or an irrelevant protein (control transfectant). Binding of HT-12D8 was detected with a PE- conjugated secondary antibody. A positive signal was obtained only with CEACAM20 transfected cells.)
Application Data
(Fig.1: FACS analysis of BOSC23 cells using HT-12D8 Cat.# GM-0516. BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM20 (red curve) or an irrelevant protein (control transfectant). Binding of HT-12D8 was detected with a PE- conjugated secondary antibody. A positive signal was obtained only with CEACAM20 transfected cells.)
NCBI and Uniprot Product Information
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Product Notes
The CEACAM20 ceacam20 (Catalog #AAA71470) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CEACAM20 monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CEACAM20 can be used in a range of immunoassay formats including, but not limited to, ELISA (Competitive ELISA), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CEACAM20 ceacam20 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CEACAM20, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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