Application Data
(Fig. 2: BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3-8 or PSG. The latter expressed as a membrane bound fusion protein. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1,3,4,5 and 6: D14HD11; CEACAM7: BAC2; CEACAM8: Tet2; PSG: BAP3; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G-purified GM-2H6 was tested on all CEACAM transfectants. A positive signal was obtained only with CEACAM8 transfected cells (red curve).)
Mouse anti-Human CEACAM8 Monoclonal Antibody | anti-CEACAM8 antibody
anti-human CEACAM8 monoclonal antibody
Gene Names
CEACAM8; CD67; CGM6; CD66b; NCA-95
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay
Purity
Protein G
Synonyms
CEACAM8, Antibody; anti-human CEACAM8 monoclonal antibody; CD66b; NCA-95; anti-CEACAM8 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1/kappa
Clone Number
GM2H6
Specificity
anti-human CEACAM8 (CD66b/NCA-95)
Purity/Purification
Protein G
Form/Format
Purified; phosphate buffered saline, pH 7.2
Sequence Length
349
Applicable Applications for anti-CEACAM8 antibody
ELISA (Competitive ELISA), FCM/FACS (Flow Cytometry)
Immunogen
Genetic immunisation with cDNA encoding human CEACAM8
Selection
Based on recognition of the complete native protein expressed on transfected mammalian cells
Preparation and Storage
Short term: 2°C - 8°C; long term: -20°C (avoid repeated freezing and thawing)
Application Data
(Fig. 2: BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3-8 or PSG. The latter expressed as a membrane bound fusion protein. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1,3,4,5 and 6: D14HD11; CEACAM7: BAC2; CEACAM8: Tet2; PSG: BAP3; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G-purified GM-2H6 was tested on all CEACAM transfectants. A positive signal was obtained only with CEACAM8 transfected cells (red curve).)
Application Data
(Fig. 2: BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3-8 or PSG. The latter expressed as a membrane bound fusion protein. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1,3,4,5 and 6: D14HD11; CEACAM7: BAC2; CEACAM8: Tet2; PSG: BAP3; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G-purified GM-2H6 was tested on all CEACAM transfectants. A positive signal was obtained only with CEACAM8 transfected cells (red curve).)
Application Data
(Fig.1: FACS analysis of BOSC23 cells using GM2H6 Cat.# AAA71467. BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM8 (red curve) or an irrelevant protein (control transfectant). Binding of AAA71467 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with CEACAM8 transfected cells.)
Application Data
(Fig.1: FACS analysis of BOSC23 cells using GM2H6 Cat.# AAA71467. BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM8 (red curve) or an irrelevant protein (control transfectant). Binding of AAA71467 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with CEACAM8 transfected cells.)
Related Product Information for anti-CEACAM8 antibody
Description: Purified monoclonal mouse antibody
Background: CEA-related cell adhesion molecule 8 (CEACAM8, CD66b) belongs to the carcinoembryonic antigen (CEA) gene family (1,3,4). It encodes a glycosylphosphatidylinositol (GPI)-linked glycoprotein with a Mr of 95,000 which is expressed in cells of the granulocyte-lineage. It is expressed in neutrophils and eosinophils and is characterized as a granulocyte-specific activation antigen. CEACAM8 could serve as a marker for granulocyte activities (2). Like all members of the CEA family, it consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by two immunoglobulin constant-like A and B domains.
Background: CEA-related cell adhesion molecule 8 (CEACAM8, CD66b) belongs to the carcinoembryonic antigen (CEA) gene family (1,3,4). It encodes a glycosylphosphatidylinositol (GPI)-linked glycoprotein with a Mr of 95,000 which is expressed in cells of the granulocyte-lineage. It is expressed in neutrophils and eosinophils and is characterized as a granulocyte-specific activation antigen. CEACAM8 could serve as a marker for granulocyte activities (2). Like all members of the CEA family, it consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by two immunoglobulin constant-like A and B domains.
References
1. Zimmermann W (2002). Carcinoembryonic antigen. In Wiley Encyclopedia of Molecular Medicine (T. Creighton, ed.), John Wiley & Sons Inc., New York, USA, pp. 459-462.
2. Zhao L, Xu S, Fjaertoft G, Pauksen K, Hakansson L and Venge P (2004). An enzyme-linked immunosorbent assay for human carcinoembryonic antigen-related cell adhesion molecule 8, a biological marker of granulocyte activities in vivo. J. Immunol. Methods 293(1-2):207-14
Hammarström S (1999). The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues. Semin. Cancer Biol. 9, 67-81.
2. Zhao L, Xu S, Fjaertoft G, Pauksen K, Hakansson L and Venge P (2004). An enzyme-linked immunosorbent assay for human carcinoembryonic antigen-related cell adhesion molecule 8, a biological marker of granulocyte activities in vivo. J. Immunol. Methods 293(1-2):207-14
Hammarström S (1999). The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues. Semin. Cancer Biol. 9, 67-81.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
carcinoembryonic antigen-related cell adhesion molecule 8
NCBI Official Synonym Full Names
carcinoembryonic antigen-related cell adhesion molecule 8
NCBI Official Symbol
CEACAM8
NCBI Official Synonym Symbols
CD67; CGM6; CD66b; NCA-95
NCBI Protein Information
carcinoembryonic antigen-related cell adhesion molecule 8; CD67 antigen; carcinoembryonic antigen CGM6; non-specific cross-reacting antigen NCA-95; carcinoembryonic antigen gene family member 6
UniProt Gene Name
CEACAM8
UniProt Synonym Gene Names
CGM6
UniProt Entry Name
CEAM8_HUMAN
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Product Notes
The CEACAM8 ceacam8 (Catalog #AAA71467) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CEACAM8 monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CEACAM8 can be used in a range of immunoassay formats including, but not limited to, ELISA (Competitive ELISA), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CEACAM8 ceacam8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CEACAM8, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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