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FCM (Flow Cytometry)
(Flow cytometric analysis of Siha cells with Cellubrevin antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Rabbit Cellubrevin Monoclonal Antibody | anti-VAMP3 antibody
Cellubrevin Antibody
Gene Names
VAMP3; CEB
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
Cellubrevin; Monoclonal Antibody; Cellubrevin Antibody; CEB antibody; Cellubrevin antibody; Synaptobrevin 3 antibody; Synaptobrevin-3 antibody; VAMP 3 antibody; VAMP-3 antibody; VAMP3 antibody; VAMP3_HUMAN antibody; Vesicle associated membrane protein 3 antibody; Vesicle-associated membrane protein 3 antibody; anti-VAMP3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JG36-31
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-VAMP3 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Synthetic peptide conjugated to KLH within N-terminal human Cellubrevin.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of Siha cells with Cellubrevin antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
FCM (Flow Cytometry)
(Flow cytometric analysis of Siha cells with Cellubrevin antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining Cellubrevin in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Cellubrevin in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Cellubrevin in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Cellubrevin in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Cellubrevin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Cellubrevin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Cellubrevin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Cellubrevin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue using anti-Cellubrevin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue using anti-Cellubrevin antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of Cellubrevin on A549 (1), 293T (2) cell lysates using anti-Cellubrevin antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of Cellubrevin on A549 (1), 293T (2) cell lysates using anti-Cellubrevin antibody at 1/1, 000 dilution.)
Related Product Information for anti-VAMP3 antibody
Vesicle-associated membrane proteins, known as VAMPs, also designated synaptobrevins, include VAMP-1, VAMP-2, VAMP-3 (cellubrevin), and synaptotagmin, a protein that may function as an inhibitor of exocytosis. VAMP proteins are vesicular factors that are important components of the machinery controlling docking and/or fusion of secretory vesicles with their target membrane. Synaptosomal-associated proteins, known as SNAPs, including alpha- and gamma-SNAP, are cytoplasmic proteins that bind to a membrane receptor complex composed of VAMP, SNAP 25 and syntaxin. Pancreatic beta-cells express VAMP-2 and VAMP-3, and either one or both of these proteins selectively control Ca2+-mediated insulin secretion. In addition, VAMP-2 and VAMP-3 are expressed on GLUT4-containing vesicle membranes isolated from 3T3-Ll adipocytes and are important components of the insulin-dependent translocation of GLUT4 to the cell surface in adipocytes.
Product Categories/Family for anti-VAMP3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
100
NCBI Official Full Name
Vesicle-associated membrane protein 3
NCBI Official Synonym Full Names
vesicle-associated membrane protein 3
NCBI Official Symbol
VAMP3
NCBI Official Synonym Symbols
CEB
NCBI Protein Information
vesicle-associated membrane protein 3; VAMP-3; cellubrevin; synaptobrevin-3
UniProt Protein Name
Vesicle-associated membrane protein 3
UniProt Gene Name
VAMP3
UniProt Synonym Gene Names
SYB3; VAMP-3; CEB
UniProt Entry Name
VAMP3_HUMAN
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Product Notes
The VAMP3 vamp3 (Catalog #AAA30492) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cellubrevin Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cellubrevin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:2000 IHC: 1:50-1:200 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the VAMP3 vamp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cellubrevin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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