<
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of K562 cells with CENPC antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Rabbit anti-Human CENPC Monoclonal Antibody | anti-CENPC antibody
CENPC Antibody
Gene Names
CENPC; MIF2; hcp-4; CENP-C; CENPC1
Reactivity
Human
Applications
Immunoprecipitation, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
ProA affinity purified
Synonyms
CENPC, Antibody; CENPC Antibody; CENP-C 1 antibody; CENP-C antibody; CENPC_HUMAN antibody; CENPC1 antibody; Centromere autoantigen C antibody; Centromere autoantigen C1 antibody; Centromere protein C 1 antibody; Centromere protein C antibody; hcp-4 antibody; ICEN7 antibody; Interphase centromere complex protein 7 antibody; MIF2 antibody; anti-CENPC antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
JG36-15
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-CENPC antibody
IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein within human CENPC aa 400-600.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of K562 cells with CENPC antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of K562 cells with CENPC antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining CENPC in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining CENPC in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-CENPC antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-CENPC antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CENPC antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CENPC antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.)
WB (Western Blot)
(Western blot analysis of CENPC on K562 cell lysate using anti-CENPC antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of CENPC on K562 cell lysate using anti-CENPC antibody at 1/1, 000 dilution.)
Related Product Information for anti-CENPC antibody
A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The evolutionarily conserved Centromere Protein-C, CENP-C, is a kinetochore assembly protein. CENP-C is located on the fibers of the kinetochore and constitutes a kinetochore organizing center that tightly associates with DNA. CENP-C is necessary for the formation of a functional centromere, which indicates that CENP-C is important for mitotic progression. In addition, CENP-C is lost from centromeres during herpes simplex virus 1 infection, causing substantial structural changes in the kinetochore, which suggests that the structure of CENP-C is regulated during the cell cycle.
Product Categories/Family for anti-CENPC antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61,544 Da
NCBI Official Full Name
Homo sapiens centromere protein C (CENPC), mRNA
NCBI Official Synonym Full Names
centromere protein C
NCBI Official Symbol
CENPC
NCBI Official Synonym Symbols
MIF2; hcp-4; CENP-C; CENPC1
NCBI Protein Information
centromere protein C; CENP-C 1; centromere autoantigen C; centromere autoantigen C1; centromere protein C 1; interphase centromere complex protein 7
UniProt Protein Name
Centromere protein C
UniProt Gene Name
CENPC
UniProt Synonym Gene Names
CENPC1; ICEN7; CENP-C; CENP-C 1
Similar Products
Product Notes
The CENPC cenpc (Catalog #AAA312655) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CENPC Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CENPC can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CENPC cenpc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CENPC, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.