Mouse anti-Human CGRP / Calcitonin Monoclonal Antibody | anti-CGRP antibody

CALCA; Alpha-type CGRP; CGRP; CGRP-I; CGRP1; CT; Calcitonin; KC; CALC1; Calcitonin 1; Katacalcin

Cat. Num. AAA52579

• Gene Names: CALCA; CT; KC; PCT; CGRP; CALC1; CGRP1; CGRP-I
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Euglobulin precipitation
• Synonyms: CGRP / Calcitonin, Antibody; CALCA; Alpha-type CGRP; CGRP; CGRP-I; CGRP1; CT; Calcitonin; KC; CALC1; Calcitonin 1; Katacalcin; Mouse Monoclonal [clone 406CT21.4.3] (IgM) to Human CGRP / Calcitonin; Human CGRP / Calcitonin; anti-CGRP antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgM
• Clone Number: 406CT21.4.3
• Specificity: This CALCA/CT antibody is generated from mice immunized with KLH conjugated synthetic peptide between 56-84 amino acids from human CALCA/CT
• Purity/Purification: Euglobulin precipitation
• Form/Format: PBS, 0.09% sodium azide
• Sequence Length: 141

• Applicable Applications for anti-CGRP antibody: WB (Western Blot), IHC (Immunohistochemistry)
• Immunogen Species: CALCA antibody was raised against Human
• Family/Subfamily: Hormone/not assigned-Hormone
• Target: CALCA
• Epitope: aa56-84
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 6 months.; For long term storage store at -20°C.

WB (Western Blot)

(CALCA/CT Antibody western blot of CEM cell line lysates (35 ug/lane). The CALCA/CT antibody detected the CALCA/CT protein (arrow).)

IHC (Immunohistochemistry)

(Anti-CGRP / Calcitonin antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

Related Product Information for anti-CGRP antibody

CALCA Antibody, Alpha-type CGRP Antibody, CGRP Antibody, CGRP-I Antibody, CGRP1 Antibody, CT Antibody, Calcitonin Antibody, KC Antibody, CALC1 Antibody, Calcitonin 1 Antibody, Katacalcin Antibody Description: CALCA encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide.

NCBI and Uniprot Product Information

• NCBI GI #: 4502545
• NCBI GeneID: 796
• NCBI Accession #: NP_001732.1
• NCBI GenBank Nucleotide #: NM_001741.2
• UniProt Accession #: P01258; Q13935; Q13937; Q52LX7; B7ZL39; P06881
• NCBI Official Full Name: calcitonin isoform CT preproprotein
• NCBI Official Synonym Full Names: calcitonin-related polypeptide alpha
• NCBI Official Symbol: CALCA
• NCBI Official Synonym Symbols: CT; KC; PCT; CGRP; CALC1; CGRP1; CGRP-I
• NCBI Protein Information: calcitonin; calcitonin gene-related peptide 1
• UniProt Protein Name: Calcitonin
• UniProt Gene Name: CALCA
• UniProt Synonym Gene Names: CALC1; CCP
• UniProt Entry Name: CALC_HUMAN