Mouse anti-Human, Mouse CHD4 Monoclonal Antibody | anti-CHD4 antibody

CHD4

Cat. Num. AAA214892

• Gene Names: CHD4; CHD-4; Mi-2b; SIHIWES; Mi2-BETA
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, Western Blot, ELISA
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: CHD4, Antibody; CHD4; ATP dependent helicase CHD4; Chromodomain helicase DNA binding protein 4; Mi 2 autoantigen 218 kDa protein; Mi 2b; Mi2 beta; anti-CHD4 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: 3F2/4
• Specificity: CHD4/Mi-2b is a component of the nucleosome remodeling and deacetylase (NuRD) complex which is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex function primarily in transcriptional repression. Accumulating evidence indicates that NuRD may regulate the transcription of specific genes by interacting with specific transcriptional factors. In addition, the NuRD complex may also participate in genome-wide transcriptional regulation through an association with histone tails.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 1912

• Applicable Applications for anti-CHD4 antibody: IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
• Positive Control: HeLa whole cell lysate or human placenta tissue lysate. Human breast carcinoma.
• Immunogen: Synthetic peptide (N- and C- terminal peptides were used for immunisation: 1. ASGLGSPSPCSAGSEEEDM & 2. CSRLANRAPEPPPQQVAQQQ) - which peptide the antibody recognises has not been tested.
• Cellular Localization: Nucleus and Cytoplasm.
• Hu-chromosome Location: 12p13.31
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

IF (Immunofluorescence)

(Confocal immunofluorescence image of MCF-7 cells using CHD4 Mouse Monoclonal Antibody (3F2/4) stained in Green (CF488) and Phalloidin is used to label the membranes.)

WB (Western Blot)

(Western Blot Analysis of SK-Br3 cell lysate using CHD4 Mouse Monoclonal Antibody (3F2/4).)

SDS-PAGE

(SDS-PAGE Analysis Purified CHD4 Mouse Monoclonal Antibody (3F2/4). Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with CHD4 Mouse Monoclonal Antibody (3F2/4).)

References

Sparmann et al. 2013. EMBO J. 32(11):1598-612.

NCBI and Uniprot Product Information

• NCBI GI #: 51599156
• NCBI GeneID: 1108
• NCBI Accession #: NP_001264.2
• NCBI GenBank Nucleotide #: NP_001264.2
• UniProt Accession #: Q14839
• Molecular Weight: 238kDa
• NCBI Official Full Name: chromodomain-helicase-DNA-binding protein 4 isoform 1
• NCBI Official Synonym Full Names: chromodomain helicase DNA binding protein 4
• NCBI Official Symbol: CHD4
• NCBI Official Synonym Symbols: CHD-4; Mi-2b; SIHIWES; Mi2-BETA
• NCBI Protein Information: chromodomain-helicase-DNA-binding protein 4
• UniProt Protein Name: Chromodomain-helicase-DNA-binding protein 4
• UniProt Gene Name: CHD4
• UniProt Synonym Gene Names: CHD-4
• UniProt Entry Name: CHD4_HUMAN