WB (Western Blot)
(Western blot analysis of HeLa cell nuclear extract (40 ug) probed with CHD5 antibody (AAA50445) at a 2 ug/ml dilution.)
Rat anti-Mouse CHD5 Monoclonal Antibody | anti-CHD5 antibody
CHD5 antibody | 5A10
Reactivity
Mouse
Applications
Western Blot, Immunofluorescence
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Synonyms
CHD5, Antibody; CHD5 antibody | 5A10; RAT ANTI MOUSE CHD5; anti-CHD5 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
5A10
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Form/Format
Purified IgG - liquid
Phosphate buffered saline
0.035% Sodium Azide (NaN3), 30% Glycerol
Phosphate buffered saline
0.035% Sodium Azide (NaN3), 30% Glycerol
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Applicable Applications for anti-CHD5 antibody
WB (Western Blot), IF (Immunofluorescence)
Species Cross-Reactivity
Human:Yes
Immunogen
A peptide within the C-terminal region of mouse CHD5
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
WB (Western Blot)
(Western blot analysis of HeLa cell nuclear extract (40 ug) probed with CHD5 antibody (AAA50445) at a 2 ug/ml dilution.)
WB (Western Blot)
(Western blot analysis of HeLa cell nuclear extract (40 ug) probed with CHD5 antibody (AAA50445) at a 2 ug/ml dilution.)
IF (Immunofluorescence)
(Immunofluorescence staining of HeLa cells (left) and mouse gut (right) with CHD5 antibody (AAA50445).)
IF (Immunofluorescence)
(Immunofluorescence staining of HeLa cells (left) and mouse gut (right) with CHD5 antibody (AAA50445).)
Related Product Information for anti-CHD5 antibody
Rat anti Mouse CHD5 antibody, clone 5A10 recognizes chromodomain-helicase-DNA-binding protein 5, also known as ATP-dependent helicase CHD5.
CHD5 is a 223 kDa member of the chromodomain helicase DNA-binding protein family of ATP-dependent chromatin remodeling complexes. Members of this family are characterized by a chromodomain, a helicase ATP-binding domain and an additional functional domain. CHD5 is a neuron-specific protein that is thought to play a role in chromatin remodeling and gene transcription (Li et al. 2014). CHD5 may also act as a tumor suppressor (Fatemi et al. 2014).
Rat anti Mouse CHD5 antibody, clone 5A10 detects a band of 260 kDa.
CHD5 is a 223 kDa member of the chromodomain helicase DNA-binding protein family of ATP-dependent chromatin remodeling complexes. Members of this family are characterized by a chromodomain, a helicase ATP-binding domain and an additional functional domain. CHD5 is a neuron-specific protein that is thought to play a role in chromatin remodeling and gene transcription (Li et al. 2014). CHD5 may also act as a tumor suppressor (Fatemi et al. 2014).
Rat anti Mouse CHD5 antibody, clone 5A10 detects a band of 260 kDa.
NCBI and Uniprot Product Information
UniProt Accession #
Similar Products
Product Notes
The CHD5 (Catalog #AAA50445) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The CHD5 antibody | 5A10 reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CHD5 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence). Researchers should empirically determine the suitability of the CHD5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHD5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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