FCM/FACS (Flow Cytometry)
(Flow cytometry analysis of CHI3L2 in HeLa cells. The cell was stained with AAA48938 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray))
Mouse anti-Human CHI3L2 Monoclonal Antibody | anti-CHI3L2 antibody
CHI3L2 antibody
Gene Names
CHI3L2; CHIL2; YKL39; YKL-39
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
CHI3L2, Antibody; CHI3L2 antibody; CHI3L2; CHIL2; YKL-39; YKL39; anti-CHI3L2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT2B5
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
390
Applicable Applications for anti-CHI3L2 antibody
FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Immunogen
Recombinant human CHI3L2 (27-390aa) purified from E. coli
Preparation and Storage
Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometry analysis of CHI3L2 in HeLa cells. The cell was stained with AAA48938 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray))
FCM/FACS (Flow Cytometry)
(Flow cytometry analysis of CHI3L2 in HeLa cells. The cell was stained with AAA48938 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray))
WB (Western Blot)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CHI3L2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: HeLa cell lysate Lane 3.: HepG2 cell lysate Lane 4.: LnCap cell lysate)
WB (Western Blot)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CHI3L2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: HeLa cell lysate Lane 3.: HepG2 cell lysate Lane 4.: LnCap cell lysate)
Related Product Information for anti-CHI3L2 antibody
CHI3L2, also known as chitinase-3-like protein 2, belongs to chitinase protein family. It is secreted by synovial fibroblasts, chondrocytes, and TGFb/IL4 stimulated monocyte derived macrophages. It has been proposed as a potential biomarker for osteoarthritis.
Product Categories/Family for anti-CHI3L2 antibody
References
Hu B., et al. (1996) J. Biol. Chem. 271: 19415-19420.; Gratchev A., et al. (2008) Biomarker Insights. 3: 39-44.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
chitinase-3-like protein 2 isoform a
NCBI Official Synonym Full Names
chitinase 3 like 2
NCBI Official Symbol
CHI3L2
NCBI Official Synonym Symbols
CHIL2; YKL39; YKL-39
NCBI Protein Information
chitinase-3-like protein 2
UniProt Protein Name
Chitinase-3-like protein 2
UniProt Gene Name
CHI3L2
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Product Notes
The CHI3L2 chi3l2 (Catalog #AAA48938) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CHI3L2 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CHI3L2 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the CHI3L2 chi3l2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHI3L2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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