Mouse CHOP Monoclonal Antibody | anti-CHOP antibody

CHOP mouse Monoclonal Antibody (2B1)

Cat. Num. AAA293801

• Gene Names: DDIT3; CHOP; CEBPZ; CHOP10; CHOP-10; GADD153
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Synonyms: CHOP, Antibody; CHOP mouse Monoclonal Antibody (2B1); DDIT3; anti-CHOP antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone Number: 2B1

• Applicable Applications for anti-CHOP antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Cellular Location: Cytoplasm. Nucleus Note=Present in the cytoplasm under non-stressed conditions and ER stress leads to its nuclear accumulation
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Application Data

(Dilution: WB 1:1000-2000, IHC 1:100-200 IF 1:200)

Application Data

(Dilution: WB 1:1000-2000, IHC 1:100-200 IF 1:200)

Application Data

(Dilution: WB 1:1000-2000, IHC 1:100-200 IF 1:200)

WB (Western Blot)

(Dilution: WB 1:1000-2000, IHC 1:100-200 IF 1:200)

Related Product Information for anti-CHOP antibody

Multifunctional transcription factor in ER stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L. Negatively regulates; expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG). Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding properties and repressing its transcriptional activity. Plays a regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response.

NCBI and Uniprot Product Information

• NCBI GI #: 304282234
• NCBI GeneID: 1649
• NCBI Accession #: NP_001181986.1
• NCBI GenBank Nucleotide #: NM_001195057.1
• UniProt Accession #: P35638
• Molecular Weight: 19,175 Da
• NCBI Official Full Name: DNA damage-inducible transcript 3 protein isoform 2
• NCBI Official Synonym Full Names: DNA-damage-inducible transcript 3
• NCBI Official Symbol: DDIT3
• NCBI Official Synonym Symbols: CHOP; CEBPZ; CHOP10; CHOP-10; GADD153
• NCBI Protein Information: DNA damage-inducible transcript 3 protein; DDIT-3; C/EBP zeta; c/EBP-homologous protein 10; CCAAT/enhancer-binding protein homologous protein; growth arrest and DNA damage-inducible protein GADD153
• UniProt Protein Name: DNA damage-inducible transcript 3 protein
• UniProt Gene Name: DDIT3
• UniProt Synonym Gene Names: CHOP; CHOP10; GADD153; DDIT-3; CHOP; CHOP-10
• UniProt Entry Name: DDIT3_HUMAN