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IF (Immunofluorescence)
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Mouse CK16 Monoclonal Antibody | anti-CK16 antibody

CK16 Monoclonal Antibody (6F6)

Gene Names
KRT16; K16; CK16; K1CP; NEPPK; FNEPPK; KRT16A
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Synonyms
CK16, Antibody; CK16 Monoclonal Antibody (6F6); Keratin type I cytoskeletal 16; KRT16; KRT16A; Keratin, type I cytoskeletal 16; Cytokeratin-16; CK-16; Keratin-16; K16; anti-CK16 antibody
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For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
6F6
Specificity
The antibody detects endogenous CK16 proteins.
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Form/Format
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Applicable Applications for anti-CK16 antibody
Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
IHC: 1:50-200
IF: 1:50-200
Immunogen
Synthetic Peptide of CK16
Modification
Unmodified/Total
Preparation and Storage
Store at -20 degree C/1 year

IF (Immunofluorescence)

(Immunofluorescence analysis of Rat-liver tissue. 1.CK16 Monoclonal Antibody(6F6)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IF (Immunofluorescence)

(Immunofluorescence analysis of Mouse-lung tissue. 1.CK16 Monoclonal Antibody(6F6)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IF (Immunofluorescence)

(Immunofluorescence analysis of Human-liver-cancer tissue. 1.CK16 Monoclonal Antibody(6F6)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1.CK16 Monoclonal Antibody(6F6) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1.CK16 Monoclonal Antibody(6F6) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(IHC staining of human gullet cancer tissue. diluted at 1:200.)

Related Product Information for anti-CK16 antibody
The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region of chromosome 17q12-q21. This keratin has been coexpressed with keratin 14 in a number of epithelial tissues, including esophagus, tongue, and hair follicles. Mutations in this gene are associated with type 1 pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus. [provided by RefSeq, Jul 2008]
Product Categories/Family for anti-CK16 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
51,268 Da
NCBI Official Full Name
keratin, type I cytoskeletal 16
NCBI Official Synonym Full Names
keratin 16
NCBI Official Symbol
KRT16
NCBI Official Synonym Symbols
K16; CK16; K1CP; NEPPK; FNEPPK; KRT16A
NCBI Protein Information
keratin, type I cytoskeletal 16; CK-16; keratin-16; cytokeratin 16; cytokeratin-16; focal non-epidermolytic palmoplantar keratoderma
UniProt Protein Name
Keratin, type I cytoskeletal 16
UniProt Gene Name
KRT16
UniProt Synonym Gene Names
KRT16A; CK-16; K16
UniProt Entry Name
K1C16_HUMAN

Similar Products

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CK16 Antibody

Host: Mouse
Reactivity: Human, Mouse, Rat
Apps: Immunohistochemistry, Immunofluorescence, Immunocytochemistry
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CK16 Antibody

Host: Mouse
Reactivity: Human, Mouse, Rat
Apps: Immunohistochemistry, Immunofluorescence

Product Notes

The CK16 krt16 (Catalog #AAA30805) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CK16 Monoclonal Antibody (6F6) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CK16 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunocytochemistry (ICC). IHC: 1:50-200 IF: 1:50-200. Researchers should empirically determine the suitability of the CK16 krt16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CK16, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.