WB (Western Blot)
(Western blot analysis of lysates from 293T GSDMB stable cells and 293T GSDMB stable cells transfected with GZMA using Cleaved Gasdermin B?C Terminal? Rabbit mAb (AAA283050) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.<b>WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS</b>)
Rabbit anti-Human Cleaved Gasdermin B Monoclonal Antibody | anti-GSDMB antibody
Cleaved Gasdermin B (C Terminal) Rabbit mAb
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity purification
Synonyms
Cleaved Gasdermin B, Antibody; Cleaved Gasdermin B (C Terminal) Rabbit mAb; GSDML; PP4052; GSDMB-1; PRO2521; Cleaved Gasdermin B (C Terminal); anti-GSDMB antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Sequence
GKSLG
Applicable Applications for anti-GSDMB antibody
ELISA, WB (Western Blot)
Immunogen
A synthetic peptide corresponding to residues surrounding Ser245 of human Gasdermin B (NP_001159430.1).
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
WB (Western Blot)
(Western blot analysis of lysates from 293T GSDMB stable cells and 293T GSDMB stable cells transfected with GZMA using Cleaved Gasdermin B?C Terminal? Rabbit mAb (AAA283050) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.<b>WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS</b>)
WB (Western Blot)
(Western blot analysis of lysates from 293T GSDMB stable cells and 293T GSDMB stable cells transfected with GZMA using Cleaved Gasdermin B?C Terminal? Rabbit mAb (AAA283050) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.<b>WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS</b>)
WB (Western Blot)
(Western blot analysis of lysates from GSDMB-C-6-his protein, using Cleaved Gasdermin B?C Terminal? Rabbit mAb (AAA283050) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 0.3s.<b>WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS</b>)
WB (Western Blot)
(Western blot analysis of lysates from GSDMB-C-6-his protein, using Cleaved Gasdermin B?C Terminal? Rabbit mAb (AAA283050) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 0.3s.<b>WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS</b>)
Related Product Information for anti-GSDMB antibody
This gene encodes a member of the gasdermin-domain containing protein family. Other gasdermin-family genes are implicated in the regulation of apoptosis in epithelial cells, and are linked to cancer. Alternative splicing and the use of alternative promoters results in multiple transcript variants. Additional variants have been described, but they are candidates for nonsense-mediated mRNA decay (NMD) and are unlikely to be protein-coding.
Product Categories/Family for anti-GSDMB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 18kDa/45kDa/46kDa/47kDa
Observed MW: 16kDa
Observed MW: 16kDa
UniProt Protein Name
Gasdermin-B
UniProt Gene Name
GSDMB
UniProt Synonym Gene Names
GSDML
UniProt Entry Name
GSDMB_HUMAN
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Product Notes
The GSDMB gsdmb (Catalog #AAA283050) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cleaved Gasdermin B (C Terminal) Rabbit mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cleaved Gasdermin B can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the GSDMB gsdmb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: GKSLG. It is sometimes possible for the material contained within the vial of "Cleaved Gasdermin B, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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