ICC (Immunocytochemistry)
(ICC staining of Collagen VI in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).)
Rabbit Collagen VI Monoclonal Antibody | anti-COL6A1 antibody
Collagen VI
Gene Names
COL6A1; OPLL; BTHLM1; UCHMD1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Protein A affinity purified
Synonyms
Collagen VI, Antibody; Collagen VI; Collagen alpha-1(VI) chain; Collagen alpha-2(VI) chain; Collagen alpha-3(VI) chain; anti-COL6A1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG
Clone Number
SD83-03
Purity/Purification
Protein A affinity purified
Form/Format
Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol / Preservative: 0.05% Sodium Azide.
Sequence Length
1028
Applicable Applications for anti-COL6A1 antibody
IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
Recombinant protein within Human Collagen VI alpha1 aa 17-255 / 1,028.
Positive Control
NIH/3T3 cell lysate, human kidney tissue lysate, rat heart tissue lysate, rat kidney tissue lysate, Hela, A549, HepG2, RH-35, human lung tissue, human liver tissue, mouse colon tissue,
human colon tissue, human skin tissue.
Subcellular Location
Extracellular matrix.
Preparation and Storage
Store at +4 degree C after thawing. Aliquot store at -20 degree C or -80 degree C. Avoid repeated freeze / thaw cycles.
ICC (Immunocytochemistry)
(ICC staining of Collagen VI in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).)
ICC (Immunocytochemistry)
(ICC staining of Collagen VI in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).)
WB (Western Blot)
(Western blot analysis of Collagen VI on diferent lysates with Rabbit anti-Collagen VI antibody at 1/2,000 dilution.Lane 1: NIH/3T3 ce l lysate (15 ug/Lane);Lane 2: Human kidney tissue lysate (20 ug/Lane);Lane 3: Rat heart tissue lysate (20 ug/Lane);Lane 4: Rat kidney tissue lysate (20 ug/Lane).Predicted band size: 109 kDa.Observed band size: 145 kDa.Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4 degree C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.)
WB (Western Blot)
(Western blot analysis of Collagen VI on diferent lysates with Rabbit anti-Collagen VI antibody at 1/2,000 dilution.Lane 1: NIH/3T3 ce l lysate (15 ug/Lane);Lane 2: Human kidney tissue lysate (20 ug/Lane);Lane 3: Rat heart tissue lysate (20 ug/Lane);Lane 4: Rat kidney tissue lysate (20 ug/Lane).Predicted band size: 109 kDa.Observed band size: 145 kDa.Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4 degree C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.)
Related Product Information for anti-COL6A1 antibody
Collagen VI (ColVI) is a type of collagen primarily associated with the extracellular matrix of skeletal muscle. ColVI maintains regularity in muscle function and stabilizes the cell membrane. It is synthesized by a complex, multistep pathway that leads to the formation of a unique network of linked microfilaments located in the extracellular matrix (ECM). ColVI plays a vital role in numerous cell types, including chondrocytes, neurons, myocytes,fibroblasts, and cardiomyocytes. ColVI molecules are made up of three alpha chains: ?1(VI),?2(VI), and ?3(VI). It is encoded by 6 genes: COL6A1, COL6A2, COL6A3, COL6A4,COL6A5, and COL6A6. The chain lengths of ?1(VI) and ?2(VI) are about 1,000 amino acids. The chain length of ?3(VI) is roughly a third larger than those of ?1(VI) and ?2(VI),and it consists of several spliced variants within the range of 2,500 to 3,100 amino acids.The first two alpha chains subunits of ColVI have a molecular weight of 140-150 KDa and the third polypeptide chain is larger with a molecular weight of 250-300kDa. ColVI is also found in the skin, lungs, blood vessels, cornea and intervertebral disc. It also forms part of the peripheral nerves, brain, myocardium and adipose tissue.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted band size: 109 kDa
NCBI Official Full Name
collagen alpha-1(VI) chain
NCBI Official Synonym Full Names
collagen type VI alpha 1 chain
NCBI Official Symbol
COL6A1
NCBI Official Synonym Symbols
OPLL; BTHLM1; UCHMD1
NCBI Protein Information
collagen alpha-1(VI) chain
UniProt Protein Name
Collagen alpha-1(VI) chain
UniProt Gene Name
COL6A1
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Product Notes
The COL6A1 col6a1 (Catalog #AAA268403) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Collagen VI reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Collagen VI can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the COL6A1 col6a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagen VI, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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