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ICC (Immunocytochemistry)
(ICC staining Collagen VI in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Rabbit Collagen VI Monoclonal Antibody | anti-COL6A1 antibody
Collagen VI Antibody
Gene Names
COL6A1; OPLL
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Purity
ProA affinity purified
Synonyms
Collagen VI; Monoclonal Antibody; Collagen VI Antibody; Alpha 1 (VI) chain (61 AA) antibody; CO6A1_HUMAN antibody; COL6A1 antibody; COL6A2 antibody; COL6A3 antibody; Collagen alpha 2 (VI) chain antibody; Collagen alpha 3 (VI) chain antibody; Collagen alpha-1 (VI) chain antibody; Collagen type VI alpha 1 antibody; Collagen type VI alpha 2 antibody; Collagen type VI alpha 3 antibody; Collagen VI alpha 1 polypeptide antibody; Collagen VI alpha 2 polypeptide antibody; Collagen VI alpha 3 polypeptide antibody; CollagenVI antibody; Human mRNA for collagen VI alpha 2 C terminal globular domain antibody; OPLL antibody; PP3610 antibody; anti-COL6A1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
SD83-03
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-COL6A1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
WB: 1:1000-1:2000
IHC: 1:50-1:200
ICC: 1:100-1:500
IHC: 1:50-1:200
ICC: 1:100-1:500
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
ICC (Immunocytochemistry)
(ICC staining Collagen VI in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Collagen VI in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Collagen VI in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Collagen VI in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Collagen VI in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Collagen VI in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Collagen VI in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Collagen VI in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Collagen VI antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Collagen VI antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Collagen VI antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Collagen VI antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Collagen VI antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Collagen VI antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of Collagen VI on different lysates using anti-Collagen VI antibody at 1/1, 000 dilution. Positive control: Lane 1: Mouse heart Lane 2: Human heart Lane 3: Human kidney)
WB (Western Blot)
(Western blot analysis of Collagen VI on different lysates using anti-Collagen VI antibody at 1/1, 000 dilution. Positive control: Lane 1: Mouse heart Lane 2: Human heart Lane 3: Human kidney)
Related Product Information for anti-COL6A1 antibody
This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.
Product Categories/Family for anti-COL6A1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
108,529 Da
NCBI Official Full Name
collagen alpha-1(VI) chain
NCBI Official Synonym Full Names
collagen, type VI, alpha 1
NCBI Official Symbol
COL6A1
NCBI Official Synonym Symbols
OPLL
NCBI Protein Information
collagen alpha-1(VI) chain; collagen alpha-1(VI) chain; alpha 1 (VI) chain (61 AA); collagen VI, alpha-1 polypeptide
UniProt Protein Name
Collagen alpha-1(VI) chain
UniProt Gene Name
COL6A1
UniProt Entry Name
CO6A1_HUMAN
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Product Notes
The COL6A1 col6a1 (Catalog #AAA30217) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Collagen VI Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Collagen VI can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC). WB: 1:1000-1:2000 IHC: 1:50-1:200 ICC: 1:100-1:500. Researchers should empirically determine the suitability of the COL6A1 col6a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagen VI, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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