Mouse Collagen VII Monoclonal Antibody | anti-COL7A1 antibody

Collagen VII

Cat. Num. AAA214899

• Gene Names: COL7A1; EBD1; EBR1; EBDCT; NDNC8
• Reactivity: Human, Sheep, Goat, Horse, Guinea Pig, Cow
• Applications: Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, Western Blot, ELISA
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Collagen VII, Antibody; Collagen VII; Alpha 1 type VII collagen; Collagen 7; Collagen alpha 1(VII) chain; Collagen type VII alpha 1; Collagen VII alpha 1 polypeptide Col7a1; EBD1; EBDCT; EBR1; Long-chain (LC) collagen; anti-COL7A1 antibody

• Host: Mouse
• Reactivity: Human, Sheep, Goat, Horse, Guinea Pig, Cow
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: LH7.2
• Specificity: Recognizes an epitope located on collagenase digested Type VII collagen (150kDa), i.e. the non-helical carboxyl terminal region of the dimer. Binding is localized at the inferior border of the lamina densa as demonstrated by immunoelectron microscopy. Type VII is also found in the retina, where its function is unknown. It interacts with Laminin 5 and Fibronectin. Collagen VII is a stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 2944

• Applicable Applications for anti-COL7A1 antibody: IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
• Positive Control: HeLa, cells. Human lymphocytes. Human lymph node or tonsil.
• Immunogen: Recombinant full-length human Collagen VII protein
• Cellular Localization: Secreted. Extracellular matrix. Basement Membrane.
• Hu-chromosome Location: 3p21.31
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

FCM/FACS (Flow Cytometry)

(Flow Cytometric Analysis of PFA-fixed HeLa cells using Collagen VII Mouse Monoclonal Antibody (LH7.2) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red))

IF (Immunofluorescence)

(Immunofluorescence Analysis of PFA-fixed HeLa cells labeling Collagen VII using Collagen VII Mouse Monoclonal Antibody (LH7.2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.)

SDS-PAGE

(SDS-PAGE Analysis Purified Collagen VII Mouse Monoclonal Antibody (LH7.2). Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human Skin stained with Collagen VII Mouse Monoclonal Antibody (LH7.2).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Skin stained with Collagen VII Mouse Monoclonal Antibody (LH7.2).)

References

Ponsioen TL et al. Mol Vis 14:652-60 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4502961
• NCBI GeneID: 1294
• NCBI Accession #: NP_000085.1
• NCBI GenBank Nucleotide #: NP_000085.1
• UniProt Accession #: Q02388
• Molecular Weight: 290kDa
• NCBI Official Full Name: collagen alpha-1(VII) chain
• NCBI Official Synonym Full Names: collagen type VII alpha 1 chain
• NCBI Official Symbol: COL7A1
• NCBI Official Synonym Symbols: EBD1; EBR1; EBDCT; NDNC8
• NCBI Protein Information: collagen alpha-1(VII) chain
• UniProt Protein Name: Collagen alpha-1(VII) chain
• UniProt Gene Name: COL7A1
• UniProt Synonym Gene Names: LC collagen
• UniProt Entry Name: CO7A1_HUMAN