Mouse Connexin 32 Monoclonal Antibody | anti-GJB1 antibody

Connexin 32 (Gap Junction Protein)

Cat. Num. AAA62996

• Gene Names: GJB1; CMTX; CX32; CMTX1
• Reactivity: Human, Mouse, Rat. Others not known.
• Applications: Immunohistochemistry
• Synonyms: Connexin 32, Antibody; Connexin 32 (Gap Junction Protein); Charcot Marie Tooth neuropathy X linked; CMTX; CMTX1; Connexin-32; Cx32; GAP junction 28kDa liver protein; Gap junction beta-1 protein; Gap junction protein beta 1 32kD; GJB1; anti-GJB1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat. Others not known.
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: GJB1/1753
• Specificity: This Ab recognizes a protein of 27-32kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane alpha-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities; therefore, one connexin usually cannot fully substitute for another.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 283

• Applicable Applications for anti-GJB1 antibody: IHC (Immunohistochemistry)
• Immunogen: Recombinant human GJB1 protein
• Cellular Localization: Cell junction, Cell membrane, Gap Junction
• Positive Control: MCF-7 Cells. Liver, Kidney, Stomach or Tonsil
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis of Purified Gap junction beta-1 protein Mouse Monoclonal Antibody (GJB1/1753). Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human Tonsil stained with Connexin 32 Monoclonal Antibody (GJB1/1753))

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Liver stained with Connexin 32 Monoclonal Antibody (GJB1/1753))

References

D. A. Goodenough et. al. Topological distribution of two connexin32 antigenic sites in intact and split rodent hepatocyte gap junctions. Journal of Cell Biology, 107(5), 1817-1824 (1988)

NCBI and Uniprot Product Information

• NCBI GI #: 4504005
• NCBI GeneID: 2705
• NCBI Accession #: NP_000157.1
• NCBI GenBank Nucleotide #: NM_000166.5
• UniProt Accession #: P08034; Q5U0S4; B2R8R2; D3DVV2
• Molecular Weight: 27-32kDa
• NCBI Official Full Name: gap junction beta-1 protein
• NCBI Official Synonym Full Names: gap junction protein beta 1
• NCBI Official Symbol: GJB1
• NCBI Official Synonym Symbols: CMTX; CX32; CMTX1
• NCBI Protein Information: gap junction beta-1 protein
• UniProt Protein Name: Gap junction beta-1 protein
• UniProt Gene Name: GJB1
• UniProt Synonym Gene Names: CX32; Cx32