IF (Immunofluorescence)
(Immunofluorescence staining of MDCK-SIAT1 cells transfected with SARS-CoV-2 NP with anti-Covid-19 & SARS-CoV Nucleoprotein antibody CR3018 (03-018) (AAA72196) Immunofluorescence analysis of MDCK-SIAT1 cells stably transfected with SARS-CoV-2 NP. The cells were seeded in a flat bottomed 96 well plate overnight, fixed in 10% formalin at 4C for 30min, permeabilised for 20min at RT and then stained with the human IgG1 version of CR3018 (03-018) in PBS/0.1% BSA at 10ug/ml for 1 hour followed by a goat anti-human Alexa Fluor 647 (Invitrogen) secondary antibody. The image is courtesy of Jack Tan, Radcliffe Department of Medicine, University of Oxford.)
Human COVID 19 Nucleocapsid (NP) Coronavirus Monoclonal Antibody | anti-Covid-19 antibody
Anti-Covid-19 & SARS-CoV Nucleoprotein [CR3018 (03-018)]
Reactivity
SARS-CoV, SARS-CoV2
Applications
Immunofluorescence, ELISA
Purity
Purified antibody. Affinity Purified using a recombinant lectin column
Synonyms
COVID 19 Nucleocapsid (NP) Coronavirus, Antibody; Anti-Covid-19 & SARS-CoV Nucleoprotein [CR3018 (03-018)]; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Nucleocapsid; Nucleoprotein (NP); NP; NC; Protein N; Nucleocapsid protein; SARS-CoV Protein N; SARS-CoV Nucleocapsid protein; SARS Coronavirus; SARS CoV 2; 2019-nCoV; anti-Covid-19 antibody
Host
Human
Reactivity
SARS-CoV, SARS-CoV2
Clonality
Monoclonal
Isotype
Human IgM, kappa
Clone Number
CR3018 (03-018)
Specificity
This antibody binds the amino acid residues between 11-19 of the N protein of the SARS CoV as well as SARS-CoV-2 (COVID-19) nucleocapsid protein.
Purity/Purification
Purified antibody. Affinity Purified using a recombinant lectin column
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-Covid-19 antibody
IF (Immunofluorescence), ELISA
Chimeric Use Statement
This reformatted human antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
PMID
15650189
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
IF (Immunofluorescence)
(Immunofluorescence staining of MDCK-SIAT1 cells transfected with SARS-CoV-2 NP with anti-Covid-19 & SARS-CoV Nucleoprotein antibody CR3018 (03-018) (AAA72196) Immunofluorescence analysis of MDCK-SIAT1 cells stably transfected with SARS-CoV-2 NP. The cells were seeded in a flat bottomed 96 well plate overnight, fixed in 10% formalin at 4C for 30min, permeabilised for 20min at RT and then stained with the human IgG1 version of CR3018 (03-018) in PBS/0.1% BSA at 10ug/ml for 1 hour followed by a goat anti-human Alexa Fluor 647 (Invitrogen) secondary antibody. The image is courtesy of Jack Tan, Radcliffe Department of Medicine, University of Oxford.)
IF (Immunofluorescence)
(Immunofluorescence staining of MDCK-SIAT1 cells transfected with SARS-CoV-2 NP with anti-Covid-19 & SARS-CoV Nucleoprotein antibody CR3018 (03-018) (AAA72196) Immunofluorescence analysis of MDCK-SIAT1 cells stably transfected with SARS-CoV-2 NP. The cells were seeded in a flat bottomed 96 well plate overnight, fixed in 10% formalin at 4C for 30min, permeabilised for 20min at RT and then stained with the human IgG1 version of CR3018 (03-018) in PBS/0.1% BSA at 10ug/ml for 1 hour followed by a goat anti-human Alexa Fluor 647 (Invitrogen) secondary antibody. The image is courtesy of Jack Tan, Radcliffe Department of Medicine, University of Oxford.)
ELISA
(Binding curve of anti-SARS-Cov-2 (COVID-19) & SARS-CoV Nucleoprotein antibody CR3018 (03-018) to SARS-CoV-2 Nucleoproteins (REC31812 and REC31851). ELISA Plate coated with SARS-CoV-2 Nucleoproteins (REC31812/REC31851; Native Antigen Company) at a concentration of 5ug/ml. A 3-fold serial dilution from 10,000 ng/ml was performed using For detection, a 1:4000 dilution of HRP-labelled anti-rabbit antibody was used. Rabbit anti-Fluorescein [4-4-20 (enhanced)] antibody was used as a control.)
ELISA
(Binding curve of anti-SARS-Cov-2 (COVID-19) & SARS-CoV Nucleoprotein antibody CR3018 (03-018) to SARS-CoV-2 Nucleoproteins (REC31812 and REC31851). ELISA Plate coated with SARS-CoV-2 Nucleoproteins (REC31812/REC31851; Native Antigen Company) at a concentration of 5ug/ml. A 3-fold serial dilution from 10,000 ng/ml was performed using For detection, a 1:4000 dilution of HRP-labelled anti-rabbit antibody was used. Rabbit anti-Fluorescein [4-4-20 (enhanced)] antibody was used as a control.)
Related Product Information for anti-Covid-19 antibody
Recombinant monoclonal antibody to Covid-19 & SARS-CoV Nucleoprotein. Recombinant Platform with variable regions (i.e. specificity) from the phage-display antibody CR3018 (03-018).
The original antibody was generated by cloning the variable regions of the scFvs selected from phage display libraries into separate vectors for IgG1 heavy-chain and light-chain expression. The harvested supernatents were then purified on protein A columns. The original immunogen was the whole irradiated virion.
The original antibody was generated by cloning the variable regions of the scFvs selected from phage display libraries into separate vectors for IgG1 heavy-chain and light-chain expression. The harvested supernatents were then purified on protein A columns. The original immunogen was the whole irradiated virion.
Product Categories/Family for anti-Covid-19 antibody
References
Van Den Brink et al. Molecular and Biological Characterization of Human Monoclonal Antibodies Binding to the Spike and Nucleocapsid Proteins of Severe Acute Respiratory Syndrome Coronavirus. Journal of Virology (2005); Vol-79 (3); p. 1635-1644.
Note on publication: Describes the generation and characterization of the antibody.
Note on publication: Describes the generation and characterization of the antibody.
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Product Notes
The Covid-19 (Catalog #AAA72196) is an Antibody produced from Human and is intended for research purposes only. The product is available for immediate purchase. The Anti-Covid-19 & SARS-CoV Nucleoprotein [CR3018 (03-018)] reacts with SARS-CoV, SARS-CoV2 and may cross-react with other species as described in the data sheet. AAA Biotech's COVID 19 Nucleocapsid (NP) Coronavirus can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA. Researchers should empirically determine the suitability of the Covid-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COVID 19 Nucleocapsid (NP) Coronavirus, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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