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WB (Western Blot)
(Western Blot; Sample: Lane1: Porcine CerebuM lysate; Lane2: 293T cell lysate; Lane3: Hela cell lysate Primary Ab: 2 ug/ml Mouse Anti-human CKB Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
Mouse Creatine Kinase B (CK-BB) Monoclonal Antibody | anti-CK-BB antibody
Monoclonal Antibody to Creatine Kinase B (CK-BB)
Gene Names
CKB; BCK; B-CK; CKBB; HEL-211; HEL-S-29
Reactivity
Human, Rat, Pig
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Affinity Chromatography
Synonyms
Creatine Kinase B (CK-BB), Antibody; Monoclonal Antibody to Creatine Kinase B (CK-BB); Creatine Kinase B; CKB; CKBB; CK-B; CK-1; Creatine Kinase, Brain; anti-CK-BB antibody
Host
Mouse
Reactivity
Human, Rat, Pig
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
D7
Specificity
The antibody is a mouse monoclonal antibody raised against CK-BB. It has been selected for its ability to recognize CK-BB in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
1mg/mL (varies by lot)
Sequence Positions
Lys11~Leu367
Applicable Applications for anti-CK-BB antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 0.5-2ug/mL
IHC: 5-20ug/mL
ICC: 5-20ug/mL
IHC: 5-20ug/mL
ICC: 5-20ug/mL
Immunogen
Recombinant Creatine Kinase B (CK-BB)
Preparation and Storage
Store at 4 degree C for frequent use. Store at -20 degree C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stable for 12 months if correctly stored.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Stable for 12 months if correctly stored.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
WB (Western Blot)
(Western Blot; Sample: Lane1: Porcine CerebuM lysate; Lane2: 293T cell lysate; Lane3: Hela cell lysate Primary Ab: 2 ug/ml Mouse Anti-human CKB Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot; Sample: Lane1: Porcine CerebuM lysate; Lane2: 293T cell lysate; Lane3: Hela cell lysate Primary Ab: 2 ug/ml Mouse Anti-human CKB Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistchemistry)
(DAB staining on IHC-P;Sample: human Stomach TissuePrimary Ab: 20ug/ml Mouse Anti-human CK-BB AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistchemistry)
(DAB staining on IHC-P;Sample: human Stomach TissuePrimary Ab: 20ug/ml Mouse Anti-human CK-BB AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: human Cardiac Muscle TissuePrimary Ab: 20ug/ml Mouse Anti-human CK-BB AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: human Cardiac Muscle TissuePrimary Ab: 20ug/ml Mouse Anti-human CK-BB AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: human CerebuM TissuePrimary Ab: 20ug/ml Mouse Anti-human CK-BB AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: human CerebuM TissuePrimary Ab: 20ug/ml Mouse Anti-human CK-BB AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: human CerebuM Tissue; Primary Ab: 20ug/ml Mouse Anti-human CK-BB AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: human CerebuM Tissue; Primary Ab: 20ug/ml Mouse Anti-human CK-BB AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: human Cardiac Muscle Tissue; Primary Ab: 20ug/ml Mouse Anti-human CK-BB AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: human Cardiac Muscle Tissue; Primary Ab: 20ug/ml Mouse Anti-human CK-BB AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Sample: human Stomach Tissue; Primary Ab: 20ug/ml Mouse Anti-human CK-BB Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Sample: human Stomach Tissue; Primary Ab: 20ug/ml Mouse Anti-human CK-BB Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
Product Categories/Family for anti-CK-BB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
381
NCBI Official Full Name
Creatine kinase B-type
NCBI Official Synonym Full Names
creatine kinase, brain
NCBI Official Symbol
CKB
NCBI Official Synonym Symbols
BCK; B-CK; CKBB; HEL-211; HEL-S-29
NCBI Protein Information
creatine kinase B-type; creatine kinase-B; creatine kinase B chain; epididymis luminal protein 211; epididymis secretory protein Li 29
UniProt Protein Name
Creatine kinase B-type
UniProt Gene Name
CKB
UniProt Synonym Gene Names
CKBB
UniProt Entry Name
KCRB_HUMAN
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Product Notes
The CK-BB ckb (Catalog #AAA21230) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Lys11~Leu367. The Monoclonal Antibody to Creatine Kinase B (CK-BB) reacts with Human, Rat, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's Creatine Kinase B (CK-BB) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 0.5-2ug/mL IHC: 5-20ug/mL ICC: 5-20ug/mL. Researchers should empirically determine the suitability of the CK-BB ckb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Creatine Kinase B (CK-BB), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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