Mouse anti-Human, mouse Creatine Kinase BB Monoclonal Antibody | anti-CKB antibody

Creatine Kinase BB Antibody (Center)

Cat. Num. AAA283599

• Gene Names: CKB; BCK; B-CK; CKBB; HEL-211; HEL-S-29
• Reactivity: Human, mouse
• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Dot Blot
• Synonyms: Creatine Kinase BB, Antibody; Creatine Kinase BB Antibody (Center); Creatine kinase B-type; B-CK; Creatine kinase B chain; CKB; CKBB; anti-CKB antibody

• Host: Mouse
• Reactivity: Human, mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 893CT29.1.1
• Specificity: Purified His-tagged CKB protein was used to produced this monoclonal antibody.
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Sequence Length: 381

• Applicable Applications for anti-CKB antibody: Cell Culture, FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), IF (Immunofluorescence), IHC (Immunohistochemistry), DB (Dot Blot)
• Function: Catalyzes the transfer of phosphate between ATP and various phosphogens. Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (Probable). Acts a key regulator of adapative thermogenesis as part of mediating phosphorylation of creatine to initiate a futile cycle of creative phosphorylation and dephosphorylation (By similarity). During the futile cycle, which dissipates the high energy charge of energy of N-phospcreatine as heat without performing any mechanical or chemical work (By similarity).
• Cellular location: Cytoplasm, cytosol [ECO:0000250|UniProtKB:Q04447] Mitochondrion {ECO:0000250|UniProtkb: Q04447} Note= Localizes to the mitochondria of thermogenic fat cells via the internal MTS-like signal (iMTS-L) region [ECO:0000250|UniProtKB: Q04447}.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(Creatine Kinase BB (CKB) Antibody (Center) western blot analysis in MDA-MB453,293,Y79 cell line,mouse stomach and brain tissue lysates (35ug/lane).This demonstrates the CKB antibody detected the CKB protein (arrow).)

Related Product Information for anti-CKB antibody

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Product Categories/Family for anti-CKB antibody

Crown Antibodies; Cancer; Cardiovascular; Cell Biology; Metabolism; Signal Transduction

References

Villarreal-Levy G., et al. Biochem. Biophys. Res. Commun. 144:1116-1127(1987).
Mariman E.C.M., et al. Genomics 1:126-137(1987).
Kaye F.J., et al. J. Clin. Invest. 79:1412-1420(1987).
Mariman E.C.M., et al. Nucleic Acids Res. 17:6385-6385(1989).
Ota T., et al. Nat. Genet. 36:40-45(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 21536286
• NCBI GeneID: 1152
• NCBI Accession #: NP_001814.2
• NCBI GenBank Nucleotide #: NM_001823.4
• UniProt Accession #: P12277; Q2LE07; Q6FG40; Q9UC66; A8K236; B2R5R4
• Molecular Weight: Calculated MW: 42644
• NCBI Official Full Name: creatine kinase B-type
• NCBI Official Synonym Full Names: creatine kinase, brain
• NCBI Official Symbol: CKB
• NCBI Official Synonym Symbols: BCK; B-CK; CKBB; HEL-211; HEL-S-29
• NCBI Protein Information: creatine kinase B-type
• UniProt Protein Name: Creatine kinase B-type
• UniProt Gene Name: CKB
• UniProt Synonym Gene Names: CKBB
• UniProt Entry Name: KCRB_HUMAN