Mouse Creatine Kinase-BB (CK-BB) Monoclonal Antibody | anti-CK-BB antibody

Creatine Kinase-BB (CK-BB)

Cat. Num. AAA62932

• Gene Names: CKB; BCK; B-CK; CKBB; CPK-B; HEL-211; HEL-S-29
• Reactivity: Human. Others not known.
• Applications: Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
• Synonyms: Creatine Kinase-BB (CK-BB), Antibody; Creatine Kinase-BB (CK-BB); B-CK; BB-CK; creatine kinase-B; Creatine kinase B chain; CKBB creatine kinase B-type; creatine kinase, brain; CKBB; Brain creatine kinase; Creatine kinase B-type; Creatine Kinase BB Isoenzyme; Creatine kinase brain; Creatine phosphokinase BB; anti-CK-BB antibody

• Host: Mouse
• Reactivity: Human. Others not known.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: CKB/1268
• Specificity: Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB. It shows minimal reactivity with other human serum proteins
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 381

• Applicable Applications for anti-CK-BB antibody: IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), ELISA
• Immunogen: Recombinant full-length human CKB protein
• Cellular Localization: Cytoplasmic
• Hu-chromosome Location: 14q32
• Positive Control: Cerebellum
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Application Data

(Analysis of Protein Array containing more than 19, 000 full-length human proteins using Creatine Kinase-B (CKB) Mouse Monoclonal Antibody (CKBB/1268). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

SDS-PAGE

(SDS-PAGE Analysis Purified Creatine Kinase-B (CKB) Mouse Monoclonal Antibody (CKB/1268). Confirmation of Integrity and Purity of Antibody.)

References

Mariman, E.C., et al. 1989. Complete nucleotide sequence of the human creatine kinase B gene. Nucleic Acids Res. 17: 6385. Mariman, E.C., et al. 1987. Structure and expression of the human creatine kinase B gene. Genomics 1: 126-137.

NCBI and Uniprot Product Information

• NCBI GI #: 21536286
• NCBI GeneID: 1152
• NCBI Accession #: NP_001814.2
• NCBI GenBank Nucleotide #: NM_001823.4
• UniProt Accession #: P12277; Q2LE07; Q6FG40; Q9UC66; A8K236; B2R5R4
• Molecular Weight: 43kDa (Monomer); 86kDa (Dimer)
• NCBI Official Full Name: creatine kinase B-type isoform 1
• NCBI Official Synonym Full Names: creatine kinase B
• NCBI Official Symbol: CKB
• NCBI Official Synonym Symbols: BCK; B-CK; CKBB; CPK-B; HEL-211; HEL-S-29
• NCBI Protein Information: creatine kinase B-type
• UniProt Protein Name: Creatine kinase B-type
• UniProt Gene Name: CKB
• UniProt Synonym Gene Names: CKBB; CPK-B