Application Data
(Fig.2: SDS-PAGE analysis of purified LV-2A2 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified LV-2A2 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.)
Mouse anti-Human Crisp3 Monoclonal Antibody | anti-CRISP3 antibody
anti-human Cysteine-rich secretory protein 3 (Crisp3) monoclonal antibody
Gene Names
CRISP3; Aeg2; CRS3; SGP28; CRISP-3; dJ442L6.3
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay
Purity
Protein G
Synonyms
Crisp3, Antibody; anti-human Cysteine-rich secretory protein 3 (Crisp3) monoclonal antibody; anti-CRISP3 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
LV-2A2
Specificity
anti-human cysteine-rich secretory protein 3 (Crisp3, SGP28)
Purity/Purification
Protein G
Form/Format
Purified
Sequence Length
268
Applicable Applications for anti-CRISP3 antibody
ELISA (Competitive ELISA), FCM/FACS (Flow Cytometry)
Buffer
phosphate buffered saline, pH 7.2
Immunogen
genetic immunisation with cDNA encoding human Crisp3
Selection
based on recognition of the complete native protein expressed on transfected
mammalian cells
Immunogen
cDNA
Preparation and Storage
Short term: 2 degree C - 8 degree C; long term: -20 degree C (avoid repeated freezing and thawing)
Application Data
(Fig.2: SDS-PAGE analysis of purified LV-2A2 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified LV-2A2 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.)
Application Data
(Fig.2: SDS-PAGE analysis of purified LV-2A2 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified LV-2A2 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.)
Application Data
(Fig.1: FACS analysis of BOSC23 cells using LV-2A2 Cat.# GM-0904. BOSC23 cells were transiently transfected with an expression vector encoding either Crisp3 (red curve) or an irrelevant protein (control transfectant: black curve). Binding of LV-2A2 was detected with a PEconjugated secondary antibody. A positive signal was obtained only with Crisp3 transfected cells.)
Application Data
(Fig.1: FACS analysis of BOSC23 cells using LV-2A2 Cat.# GM-0904. BOSC23 cells were transiently transfected with an expression vector encoding either Crisp3 (red curve) or an irrelevant protein (control transfectant: black curve). Binding of LV-2A2 was detected with a PEconjugated secondary antibody. A positive signal was obtained only with Crisp3 transfected cells.)
Related Product Information for anti-CRISP3 antibody
Background: Cysteine-rich secretory protein 3 (CRISP3) belongs to the cysteine-rich secretory protein family. CRISPs are characterized by a cysteine-rich domain at the COOH terminal that form 8 intramolecular disulfide bonds (1). Mammalian members of the CRISP family are expressed predominantly in the male reproductive tract and are implicated in the process of reproduction from spermiogenesis, posttesticular sperm maturation and capacitation to oocyte-sperm fusion (2). CRISP3 is epithelium-specific and found predominantly in salivary gland, pancreas and prostate, and in less abundance in the epididymis, ovary, thymus and colon. CRISP3 is up-regulated in malignant prostatic epithelium, therefore, it can be used as a potential prostate cancer biomarker (3).
References
Krätzschmar J, Haendler B, Eberspaecher U, Roosterman D, Donner P, Schleuning WD (1996). The human cysteine-rich secretory protein (CRISP) family. Primary structure and tissue distribution of CRISP-1, CRISP-2 and CRISP-3 Eur J Biochem 15;236(3):827-36
Udby L, Bjartell A, Malm J, Egesten A, Lundwall A, Cowland JB, Borregaard N, Kjeldsen L (2005). Characterization and Localization of Cysteine-Rich Secretory Protein 3 (CRISP-3) in the Human Male Reproductive Tract. J Androl ;26(3):333-42
Kosari F, Asmann YW, Cheville JC and Vasmatzis G (2002). Cysteine-rich Secretory Protein- 3: A Potential Biomarker for Prostate Cancer. Cancer Epidemiology Biomarkers & Prevention 11;1419-1426
Udby L, Bjartell A, Malm J, Egesten A, Lundwall A, Cowland JB, Borregaard N, Kjeldsen L (2005). Characterization and Localization of Cysteine-Rich Secretory Protein 3 (CRISP-3) in the Human Male Reproductive Tract. J Androl ;26(3):333-42
Kosari F, Asmann YW, Cheville JC and Vasmatzis G (2002). Cysteine-rich Secretory Protein- 3: A Potential Biomarker for Prostate Cancer. Cancer Epidemiology Biomarkers & Prevention 11;1419-1426
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,116 Da
NCBI Official Full Name
cysteine-rich secretory protein 3 isoform 2
NCBI Official Synonym Full Names
cysteine-rich secretory protein 3
NCBI Official Symbol
CRISP3
NCBI Official Synonym Symbols
Aeg2; CRS3; SGP28; CRISP-3; dJ442L6.3
NCBI Protein Information
cysteine-rich secretory protein 3; cysteine-rich secretory protein-3; specific granule protein (28 kDa); specific granule protein of 28 kDa
UniProt Gene Name
CRISP3
UniProt Synonym Gene Names
CRISP-3; SGP28
UniProt Entry Name
CRIS3_HUMAN
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Product Notes
The CRISP3 crisp3 (Catalog #AAA71471) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human Cysteine-rich secretory protein 3 (Crisp3) monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Crisp3 can be used in a range of immunoassay formats including, but not limited to, ELISA (Competitive ELISA), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CRISP3 crisp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Crisp3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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