Mouse CRYPTOSPORIDIUM Monoclonal Antibody

MOUSE ANTI CRYPTOSPORIDIUM:FITC

Cat. Num. AAA49038

• Applications: Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: CRYPTOSPORIDIUM, Antibody; MOUSE ANTI CRYPTOSPORIDIUM:FITC; anti-CRYPTOSPORIDIUM antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG3
• Clone Number: BEL 0126
• Specificity: Mouse anti Cryptosporidium antibody, clone BEL 0126 recognizes a membrane antigen expressed by the oocysts of Cryptosporidium sp., an obligate enteric coccidian parasite of the phylum Apicomplexa that infects the gastrointestinal tract. The parasite is one of the most important enteric pathogens in both humans and animals (Rose et al. 2002). Since its first in 1975 (Meisel et al. 1976), Cryptosporidiosis, has become one of the most prominent public health concerns worldwide (Rose et al. 2002). Cryptosporidium oocysts are resistant to chlorine and their small size makes removal by filtration difficult. Alternative methods have been developed such as UV and ozone treatment alongside monitoring using Immunofluorescence screening (Rose et al. 2002). Cryptosporidiosis is a disease affecting the intestines of mammals which is spread through the fecal-oral route. The main symptom of is self-limiting diarrhea in people with intact immune systems. However, in immunocompromised individuals, such as AIDS patients, infection can cause permanent & life-threatening diarrhea (Ma et al. 1984).
• Form/Format: Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
• Concentration: IgG concentration 0.1mg/ml (varies by lot)

• Applicable Applications for anti-CRYPTOSPORIDIUM antibody: IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Target Species: Protozoan
• Preparation: Purified IgG prepared by affinity chromatography on Protein G
• Buffer Solution: Phosphate buffered saline
• Preservative Stabilizers: 0.09% Sodium Azide (NaN₃); 1% Bovine serum albumin
• Immunogen: Purified cryptosporidium oocysts from bovine faeces.
• Max Ex/Em: Fluorophore: FITC; Excitation Max (nm): 490; Emission Max (nm): 525
• Preparation and Storage: Store at +4°C or at -20°C if preferred.; Storage in frost-free freezers is not recommended.; This product should be stored undiluted. This product is photosensitive and should be protected from light.; Avoid repeated freezing and thawing as this may denature the antibody. ; Should this product contain a precipitate we recommend microcentrifugation before use.